Lemonakis Nikolaos, Poudyal Hemant, Halabalaki Maria, Brown Lindsay, Tsarbopoulos Anthony, Skaltsounis Alexios-Leandros, Gikas Evagelos
Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens 11527, Greece.
School of Health and Wellbeing, University of Southern Queensland, Toowoomba 4350, Australia; Department of Diabetes, Endocrinology and Nutrition and The Hakubi Centre for Advanced Research, Kyoto University, Kyoto, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jan 15;1041-1042:45-59. doi: 10.1016/j.jchromb.2016.12.020. Epub 2016 Dec 15.
Hydroxytyrosol (HT), an important component of olive fruit and olive oil, improves the signs of metabolic syndrome in rats following chronic treatment. At a dose of 20mg/kg/day, HT decreased adiposity and improved cardiovascular and liver structure and function in rats fed with a high-carbohydrate, high-fat diet. An untargeted metabolomics approach has been employed using both UPLC-Orbitrap and -QqTOF methods to identify the changes induced by chronic HT administration on the plasma metabolome. 31 metabolites have been found to be differentially expressed between the examined groups. HT was shown to decrease biosynthesis of unsaturated fatty acids, fatty acid biosynthesis, and the metabolism of linoleic acid, retinol, sphingolipids and arachidonic acid, whereas glycerolipid metabolism is up-regulated. These are plausible mechanisms for the attenuation by HT of cardiovascular, liver and metabolic changes in high-carbohydrate, high-fat diet fed rats.
羟基酪醇（HT）是橄榄果实和橄榄油的重要成分，长期治疗后可改善大鼠代谢综合征的症状。以20mg/kg/天的剂量，HT可降低高碳水化合物、高脂肪饮食喂养大鼠的肥胖程度，并改善其心血管和肝脏结构及功能。采用非靶向代谢组学方法，使用超高效液相色谱-轨道阱和-四极杆飞行时间串联质谱法来鉴定长期给予HT对血浆代谢组的影响。已发现31种代谢物在各检测组之间存在差异表达。结果表明，HT可减少不饱和脂肪酸的生物合成、脂肪酸生物合成以及亚油酸、视黄醇、鞘脂和花生四烯酸的代谢，而甘油脂质代谢则上调。这些可能是HT减轻高碳水化合物、高脂肪饮食喂养大鼠心血管、肝脏和代谢变化的机制。
