Chen Ming-Mao, Cao Huan, Liu Yuan-Yuan, Liu Yan, Song Fei-Fei, Chen Jing-Di, Zhang Qi-Qing, Yang Wen-Zhi
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
State Key Lab of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
Colloids Surf B Biointerfaces. 2017 Mar 1;151:189-195. doi: 10.1016/j.colsurfb.2016.05.045. Epub 2016 May 17.
Wound treatment should meet the challenge both of preventing infection and promoting wound healing. To design a sequential delivery system for wound healing, PLGA-glycol chitosan (GC) core-shell microspheres containing chlorhexidine acetate (CHA) at the GC shell and bFGF in the core of PLGA microspheres were fabricated using emulsion-solvent evaporation method. SEM showed that the microspheres were all spherical in shape with a smooth surface. The average size of PLGA-GC microspheres increased due to the GC coating on the surface. The results of release profiles and fluorescence images indicated that PLGA-GC microspheres had an ability to deliver drugs in sequence. The CHA was rapidly released, whereas the proteins presented a sustained release. The release behavior could be modulated by changing the GC amount. Antibacterial assay and cell proliferation tests suggested that the released CHA and bFGF retained their antimicrobial activity and bioactivity during preparation. The microspheres exhibited non-cytotoxicity against 3T3 cells and had a good biocompatibility. These results demonstrated that PLGA-GC core-shell microspheres could be a promising controlled release system of delivering drugs and proteins in sequence for wound healing.
伤口治疗应应对预防感染和促进伤口愈合这两个挑战。为了设计一种用于伤口愈合的顺序递送系统，采用乳液-溶剂蒸发法制备了PLGA-壳聚糖二醇（GC）核壳微球，其GC壳层含有醋酸氯己定（CHA），PLGA微球核心含有碱性成纤维细胞生长因子（bFGF）。扫描电子显微镜显示微球均呈球形，表面光滑。由于表面的GC涂层，PLGA-GC微球的平均尺寸增大。释放曲线和荧光图像结果表明，PLGA-GC微球具有顺序递送药物的能力。CHA迅速释放，而蛋白质呈现持续释放。释放行为可通过改变GC量来调节。抗菌试验和细胞增殖试验表明，释放的CHA和bFGF在制备过程中保留了它们的抗菌活性和生物活性。微球对3T3细胞表现出无细胞毒性，并且具有良好的生物相容性。这些结果表明，PLGA-GC核壳微球可能是一种有前途的用于伤口愈合的顺序递送药物和蛋白质的控释系统。
