Valenzuela J R, Sethi A K, Aulik N A, Poulsen K P
Wisconsin Veterinary Diagnostic Laboratory, Madison 53706.
Department of Population Health Sciences, University of Wisconsin-Madison, Madison 53726.
J Dairy Sci. 2017 Feb;100(2):1319-1330. doi: 10.3168/jds.2016-11419. Epub 2016 Dec 21.
Salmonellosis on the dairy continues to have a significant effect on animal health and productivity and in the United States. Additionally, Salmonella enterica ssp. enterica causes an estimated 1.2 million cases of human illness annually. Contributing to the morbidity and mortality in both human and domestic animal species is emergence of antimicrobial resistance by Salmonella species and increased incidence of multidrug-resistant isolates. This study describes serotype distribution and the antimicrobial resistance patterns for various Salmonella serotypes isolated from bovine samples submitted to the Wisconsin Veterinary Diagnostic Laboratory (WVDL) over the past 10 yr. Salmonella serotyping and antimicrobial susceptibility testing data were obtained from the laboratory information management system at WVDL. Data from accessions were limited to bovine samples submitted to the WVDL between January 2006 and June 2015 and those that had both a definitive serotype and complete results for antimicrobial susceptibility testing. A total of 4,976 isolates were identified. Salmonella enterica ser. Dublin was the most prevalent serotype identified among bovine samples submitted to the WVDL, accounting for a total of 1,153 isolates (23% of total isolates) over the study period. Along with Dublin, Salmonella enterica ser. Cerro (795, 16%), Newport (720, 14%), Montevideo (421, 8%), Kentucky (419, 8%), and Typhimurium (202, 4%) comprised the top 6 most commonly isolated serotypes during that time. Overall, resistance of bovine Salmonella isolates in the study population remained stable, although decreases in resistance were noted for gentamicin, neomycin, and trimethoprim sulfamethoxazole during the study period. All isolates remained susceptible to enrofloxacin. These data show that antimicrobial susceptibility for bovine Salmonella has changed in the population served by WVDL in the past 10 yr. This information is important for understanding Salmonella disease ecology in Wisconsin. Our findings are also relevant for animal and public health by improving informed antimicrobial use, new drug development, and regulation of their use in food animals.
奶牛场的沙门氏菌病继续对美国的动物健康和生产力产生重大影响。此外，肠炎沙门氏菌亚种肠炎每年估计导致120万例人类疾病。沙门氏菌属出现抗菌药物耐药性以及多重耐药菌株的发病率增加，导致了人类和家畜物种的发病和死亡。本研究描述了过去10年提交给威斯康星兽医诊断实验室（WVDL）的牛样本中分离出的各种沙门氏菌血清型的血清型分布和抗菌药物耐药模式。沙门氏菌血清分型和抗菌药物敏感性测试数据来自WVDL的实验室信息管理系统。入档数据仅限于2006年1月至2015年6月提交给WVDL的牛样本，以及那些具有明确血清型和完整抗菌药物敏感性测试结果的样本。共鉴定出4976株菌株。肠炎沙门氏菌都柏林血清型是提交给WVDL的牛样本中最常见的血清型，在研究期间共有1153株（占总分离株的23%）。除都柏林血清型外，肠炎沙门氏菌塞罗血清型（795株，占16%）、纽波特血清型（720株，占14%）、蒙得维的亚血清型（421株，占8%）、肯塔基血清型（419株，占8%）和鼠伤寒血清型（202株，占4%）是当时最常分离出的6种血清型。总体而言，研究人群中牛源沙门氏菌分离株的耐药性保持稳定，尽管在研究期间庆大霉素、新霉素和甲氧苄啶磺胺甲恶唑的耐药性有所下降。所有分离株对恩诺沙星仍敏感。这些数据表明，在过去10年中，WVDL所服务人群中牛源沙门氏菌的抗菌药物敏感性发生了变化。这些信息对于了解威斯康星州的沙门氏菌病生态很重要。我们的研究结果对于动物和公共卫生也具有相关性，有助于改善抗菌药物的明智使用、新药开发以及对食用动物使用抗菌药物的监管。
