Year-Long Prevalence and Antibiotic Resistance Profiles of Serogroups Isolated from a Wisconsin Dairy Farm.

infections can significantly impact the health and productivity of dairy cattle. Asymptomatic carriage of can make it difficult to identify and monitor this pathogen across a herd. Therefore, a more focused census on dairy farms is needed to better understand the dynamics of asymptomatic carriage. Here, we monitored the prevalence of on a dairy operation in Wisconsin, USA. Fecal samples were collected over 12 months from cattle and the farm environment, subjected to isolation, serogrouped, and tested for antibiotic resistance. was highly prevalent on this farm, with an average of 90% of the cattle being carriers. Total recovery of from environmental samples ranged from 40 to 90%. Four serogroups were identified on the farm, with K being most common in cattle and C being most common in the environment. Antibiotic resistance was tested against eight antibiotics and was found to be highest for neomycin (44.5%) and sulfadimethoxine (86.3%). Our data show that serogroups associated with asymptomatic carriages are persistent and highly prevalent, with niche specificity to different locations. These results provide useful information for studying within-herd transmission of and contributes to our understanding of transmission risks within the farm ecosystem.