Deblois Courtney L, Tu Andrew D J, Scheftgen Andrew J, Suen Garret
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Microbiology Doctoral Training Program, Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Pathogens. 2024 Nov 22;13(12):1031. doi: 10.3390/pathogens13121031.
infections can significantly impact the health and productivity of dairy cattle. Asymptomatic carriage of can make it difficult to identify and monitor this pathogen across a herd. Therefore, a more focused census on dairy farms is needed to better understand the dynamics of asymptomatic carriage. Here, we monitored the prevalence of on a dairy operation in Wisconsin, USA. Fecal samples were collected over 12 months from cattle and the farm environment, subjected to isolation, serogrouped, and tested for antibiotic resistance. was highly prevalent on this farm, with an average of 90% of the cattle being carriers. Total recovery of from environmental samples ranged from 40 to 90%. Four serogroups were identified on the farm, with K being most common in cattle and C being most common in the environment. Antibiotic resistance was tested against eight antibiotics and was found to be highest for neomycin (44.5%) and sulfadimethoxine (86.3%). Our data show that serogroups associated with asymptomatic carriages are persistent and highly prevalent, with niche specificity to different locations. These results provide useful information for studying within-herd transmission of and contributes to our understanding of transmission risks within the farm ecosystem.
感染会对奶牛的健康和生产力产生重大影响。[病原体名称]的无症状携带使得在整个牛群中识别和监测这种病原体变得困难。因此，需要在奶牛场进行更有针对性的普查，以更好地了解无症状携带的动态情况。在此，我们监测了美国威斯康星州一个奶牛场中[病原体名称]的流行情况。在12个月的时间里，从牛和农场环境中采集粪便样本，进行[病原体名称]分离、血清分型，并检测抗生素耐药性。[病原体名称]在这个农场中高度流行，平均90%的牛为携带者。从环境样本中[病原体名称]的总回收率在40%至90%之间。在该农场鉴定出四个血清型，K型在牛中最为常见，C型在环境中最为常见。针对八种抗生素测试了抗生素耐药性，发现对新霉素（44.5%）和磺胺二甲氧嘧啶（86.3%）的耐药性最高。我们的数据表明，与无症状携带相关的血清型持续存在且高度流行，对不同位置具有生态位特异性。这些结果为研究[病原体名称]在牛群内的传播提供了有用信息，并有助于我们了解农场生态系统内的传播风险。
