Nanomaterials and System Lab, Department of Advanced Convergence Technology and Science, Jeju National University, Jeju 690-756, Republic of Korea.
Nanomaterials and System Lab, Department of Mechanical Engineering, Jeju National University, Jeju 690-756, Republic of Korea.
Biosens Bioelectron. 2017 May 15;91:203-210. doi: 10.1016/j.bios.2016.12.006. Epub 2016 Dec 6.
Simple, novel, and direct detection of clinically important biomolecules have continuous demand among scientific community as well as in market. Here, we report the first direct detection and facile fabrication of a cysteine-responsive, film-based, self-powered device. NH functionalized BaTiO nanoparticles (BT-NH NPs) suspended in a three-dimensional matrix of an agarose (Ag) film, were used for cysteine detection. BaTiO nanoparticles (BT NPs) semiconducting as well as piezoelectric properties were harnessed in this study. The changes in surface charge properties of the film with respect to cysteine concentrations were determined using a current-voltage (I-V) technique. The current response increased with cysteine concentration (linear concentration range=10µM-1mM). Based on the properties of the composite (BT/Ag), we created a self-powered cysteine sensor in which the output voltage from a piezoelectric nanogenerator was used to drive the sensor. The potential drop across the sensor was measured as a function of cysteine concentrations. Real-time analysis of sensor performance was carried out on urine samples by non-invasive method. This novel sensor demonstrated good selectivity, linear concentration range and detection limit of 10µM; acceptable for routine analysis.
简单、新颖、直接检测临床上重要的生物分子，这是科学界和市场的持续需求。在这里，我们报告了第一个直接检测和简便制造的半胱氨酸响应的、基于薄膜的自供电设备。NH 功能化的 BaTiO 纳米粒子 (BT-NH NPs) 悬浮在琼脂糖 (Ag) 薄膜的三维基质中，用于半胱氨酸检测。本研究利用了 BaTiO 纳米粒子 (BT NPs) 的半导体和压电特性。使用电流-电压 (I-V) 技术确定了薄膜表面电荷特性随半胱氨酸浓度的变化。电流响应随半胱氨酸浓度增加而增加 (线性浓度范围=10µM-1mM)。基于复合材料 (BT/Ag) 的特性，我们创建了一个自供电半胱氨酸传感器，其中压电纳米发电机的输出电压用于驱动传感器。传感器两端的电势差作为半胱氨酸浓度的函数进行测量。通过非侵入性方法在尿液样本上实时分析传感器性能。这种新型传感器表现出良好的选择性、线性浓度范围和 10µM 的检测限; 可用于常规分析。
