Chen Chong-You, Chen Chia-Lin, Wang Chang-Ming, Liao Wei-Ssu
Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
Nanomaterials (Basel). 2018 Feb 9;8(2):97. doi: 10.3390/nano8020097.
Paper-based analytical devices are an emerging class of lightweight and simple-to-use analytical platform. However, challenges such as instrumental requirements and chemical reagents durability, represent a barrier for less-developed countries and markets. Herein, we report an advanced laminated device using red emitting copper nanocluster and RGB digital analysis for signal improvement. Upon RGB system assistance, the device signal-to-background ratio and the calibration sensitivity are highly enhanced under a filter-free setup. In addition, the calibration sensitivity, limit of detection, and coefficient of determination are on par with those determined by instrumental fluorescence analysis. Moreover, the limitation of using oxidation-susceptible fluorescent nanomaterials is overcome by the introduction of protecting tape barriers, antioxidative sheets, and lamination enclosing. The robustness of device is highly advanced, and the durability is prolonged to more than tenfold.
纸质分析设备是一类新兴的轻便且易于使用的分析平台。然而，诸如仪器要求和化学试剂耐久性等挑战，对欠发达国家和市场来说是一个障碍。在此，我们报告一种先进的层压设备，其使用发红光的铜纳米簇和RGB数字分析来改善信号。在RGB系统的辅助下，该设备在无滤光片设置下的信背比和校准灵敏度得到了极大提高。此外，校准灵敏度、检测限和决定系数与仪器荧光分析所测定的相当。而且，通过引入保护胶带屏障、抗氧化片和层压封装，克服了使用易氧化荧光纳米材料的局限性。该设备的稳健性得到了极大提升，耐久性延长至十倍以上。
