School of Chemical Engineering and Analytical Science, The University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom.
School of Chemical Engineering and Analytical Science, The University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom.
Bioresour Technol. 2017 Mar;227:35-43. doi: 10.1016/j.biortech.2016.12.055. Epub 2016 Dec 18.
Lignocellulosic materials, mostly from agricultural and forestry residues, provide a potential renewable resource for sustainable biorefineries. Reducing sugars can be produced only after a pre-treatment stage, which normally involves chemicals but can be biological. In this case, two steps are usually necessary: solid-state cultivation of fungi for deconstruction, followed by enzymatic hydrolysis using cellulolytic enzymes. In this research, the utilisation of solid-state bioprocessing using the fungus Trichoderma longibrachiatum was implemented as a simultaneous microbial pretreatment and in-situ enzyme production method for fungal autolysis and further enzyme hydrolysis of fermented solids. Suspending the fermented solids in water at 50°C led to the highest hydrolysis yields of 226mg/g reducing sugar and 7.7mg/g free amino nitrogen (FAN). The resultant feedstock was shown to be suitable for the production of various products including ethanol.
木质纤维素材料主要来自农业和林业残余物，为可持续生物精炼厂提供了一种潜在的可再生资源。只有在预处理阶段之后才能生产还原糖，该阶段通常涉及化学物质，但也可以是生物方法。在这种情况下，通常需要两个步骤：真菌的固态培养进行解构，然后使用纤维素酶进行酶解。在这项研究中，采用固态生物加工方法，利用长枝木霉（Trichoderma longibrachiatum）作为同时进行微生物预处理和原位酶生产的方法，用于真菌自溶和进一步发酵固体的酶水解。将发酵固体悬浮在 50°C 的水中可实现最高的水解产率，分别为 226mg/g 的还原糖和 7.7mg/g 的游离氨基酸氮（FAN）。结果表明，该原料适合生产各种产品，包括乙醇。
