温度对甘蔗渣木质素上纤维素酶吸附的影响。
Temperature dependent cellulase adsorption on lignin from sugarcane bagasse.
机构信息
Sao Paulo State University-Unesp, IBILCE, São José do Rio Preto, São Paulo, Brazil; Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN, USA.
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN, USA; Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA.
出版信息
Bioresour Technol. 2018 Mar;252:143-149. doi: 10.1016/j.biortech.2017.12.061. Epub 2017 Dec 20.
Extents of adsorption of cellulolytic enzymes on lignin, derived from sugarcane bagasse, were an inverse function of incubation temperature and varied with type of lignin extraction. At 45 °C, lignin derived from acid hydrolyzed liquid hot water pretreated bagasse completely adsorbed cellulolytic enzymes from Trichoderma reesei within 90 min. Lignin derived from enzyme hydrolyzed liquid hot water pretreated bagasse adsorbed only 60% of T. reesei endoglucanase, exoglucanase and β-glucosidase activities. β-Glucosidase from Aspergillus niger was not adsorbed. At 30 °C, adsorption of all of the enzymes was minimal and enzyme hydrolysis at 30 °C approached that at 45 °C after 168 h. Hence, temperature provided an approach to decrease loss of enzyme activity by reducing enzyme adsorption on lignin. This helps to explain why simultaneous saccharification and fermentation (SSF) and consolidated bioprocessing (CBP), both carried out at 30-32 °C, could offer viable options for mitigating lignin-derived inhibition effects.
纤维素酶在来源于甘蔗渣的木质素上的吸附程度是孵育温度的反函数，并随木质素提取类型而变化。在 45°C 下，来源于酸水解液体热预处理蔗渣的木质素在 90 分钟内完全吸附了里氏木霉的纤维素酶。来源于酶水解液体热预处理蔗渣的木质素仅吸附了 60%的里氏木霉内切葡聚糖酶、外切葡聚糖酶和β-葡萄糖苷酶活性。黑曲霉的β-葡萄糖苷酶未被吸附。在 30°C 下，所有酶的吸附都很小，并且在 168 小时后，30°C 下的酶水解接近 45°C 下的酶水解。因此，温度提供了一种通过减少酶对木质素的吸附来降低酶活性损失的方法。这有助于解释为什么同时糖化和发酵（SSF）和整合生物加工（CBP）都在 30-32°C 下进行，可以提供可行的选择来减轻木质素衍生的抑制作用。
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