Zhang Huijie, Zhang Shulan
Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, No.36 Sanhao St, Heping District, Shenyang, 110004, Liaoning, China.
Arch Gynecol Obstet. 2017 Mar;295(3):705-712. doi: 10.1007/s00404-016-4277-5. Epub 2016 Dec 24.
To explore the expression of forkhead/winged helix transcription factor p3(Foxp3) and toll-like receptor 4(TLR4) in cervical cancer and evaluate their clinical significance.
Foxp3 and TLR4 protein expression was detected in 105 cervical tissue specimens including cervical cancer, cervical intraepithelial neoplasia (CIN), and healthy control samples using immunohistochemistry. Their relationship with clinicopathologic parameters was also determined.
Foxp3 and TLR4 had high levels of expression in cervical cancer cells (91.43 and 82.86%, respectively). Foxp3 levels were significantly associated with FIGO stage (P < 0.001) and tumor size (P = 0.034), while TLR4 levels were associated with clinical FIGO stage (P = 0.033) and lymph node metastasis (P = 0.031). Their expression levels were not correlated with age, histologic type, or differentiation (all P > 0.05). These findings suggest that Foxp3 and TLR4 may be useful prognostic indicators of cervical carcinoma. In addition, there were significant positive relationships between Foxp3 and TLR4 expression (r = 0.703, P < 0.001), which shows a possible link and synergistic role of Foxp3 and TLR4 in promoting the immune escape of cervical cancer.
Foxp3 and TLR4 may be useful biomarkers for patient prognosis and cervical cancer prediction and treatment.
探讨叉头/翼状螺旋转录因子p3（Foxp3）和Toll样受体4（TLR4）在宫颈癌中的表达情况，并评估其临床意义。
采用免疫组织化学法检测105例宫颈组织标本（包括宫颈癌、宫颈上皮内瘤变（CIN）和健康对照样本）中Foxp3和TLR4蛋白的表达。同时确定它们与临床病理参数的关系。
Foxp3和TLR4在宫颈癌细胞中表达水平较高（分别为91.43%和82.86%）。Foxp3水平与国际妇产科联盟（FIGO）分期（P<0.001）和肿瘤大小（P=0.034）显著相关，而TLR4水平与FIGO临床分期（P=0.033）和淋巴结转移（P=0.031）相关。它们的表达水平与年龄、组织学类型或分化程度均无相关性（均P>0.05）。这些结果表明，Foxp3和TLR4可能是宫颈癌有用的预后指标。此外，Foxp3和TLR4表达之间存在显著正相关（r=0.703，P<0.001），这表明Foxp3和TLR4在促进宫颈癌免疫逃逸方面可能存在联系和协同作用。
Foxp3和TLR4可能是患者预后及宫颈癌预测和治疗的有用生物标志物。
