Luo Qingshuang, Zhang Shulan, Wei Heng, Pang Xiaoao, Zhang Huijie
Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University Liaoning Province, China.
Int J Clin Exp Pathol. 2015 Aug 1;8(8):8717-30. eCollection 2015.
This study aimed to evaluate the relationship between forkhead box P3 (Foxp3) expression and clinicopathological characteristics of cervical cancer and to explore the influence of Foxp3 on the biological behaviors of cervical cancer cells.
In this study, immunohistochemistry, lentivirus mediated transfection, Transwell assay; CCK-8 assay, real-time PCR and flow cytometry were employed to confirm the roles of Foxp3 in the occurrence and development of cervical cancer.
Foxp3 and p16(INK4a) were highly expressed in the cervical cancer and their expressions were related to the FIGO stage, tumor size, lymph node metastasis and serum SCC. Foxp3 had a high expression in the cervical cancer cells, tumor interstitium and metastatic lymph nodes. Foxp3 expression was positively related to p16(INK4a) expression in the cervical cancer. Foxp3 expression in the cervical cancer was negatively related to the prognosis: high Foxp3 expression predicted a poor prognosis. Silencing of Foxp3 was able to inhibit the proliferation and invasiveness of cervical cancer cells, promote their apoptosis, and induce the change in cell cycle. Silencing of Foxp3 also reduced the mRNA and protein expressions of p16(INK4a) in cervical cancer cells.
Foxp3 is highly expressed in the cervical cancer, and able to facilitate the proliferation and invasiveness of cervical cancer, change cell cycle and inhibit their apoptosis, resulting in the occurrence, development and metastasis of cervical cancer.
本研究旨在评估叉头框蛋白P3（Foxp3）表达与宫颈癌临床病理特征之间的关系，并探讨Foxp3对宫颈癌细胞生物学行为的影响。
在本研究中，采用免疫组织化学、慢病毒介导的转染、Transwell实验、CCK-8实验、实时荧光定量PCR和流式细胞术来证实Foxp3在宫颈癌发生发展中的作用。
Foxp3和p16（INK4a）在宫颈癌中高表达，且它们的表达与国际妇产科联盟（FIGO）分期、肿瘤大小、淋巴结转移及血清鳞状细胞癌抗原（SCC）相关。Foxp3在宫颈癌细胞、肿瘤间质及转移淋巴结中高表达。宫颈癌中Foxp3表达与p16（INK4a）表达呈正相关。宫颈癌中Foxp3表达与预后呈负相关：Foxp3高表达预示预后不良。沉默Foxp3能够抑制宫颈癌细胞的增殖和侵袭能力，促进其凋亡，并诱导细胞周期改变。沉默Foxp3还降低了宫颈癌细胞中p16（INK4a）的mRNA和蛋白表达。
Foxp3在宫颈癌中高表达，能够促进宫颈癌的增殖和侵袭，改变细胞周期并抑制其凋亡，从而导致宫颈癌的发生、发展和转移。
