HMGCR 抑制 PCV2 感染的早期阶段，而 PKC 则增强感染的晚期阶段。

HMGCR inhibits the early stage of PCV2 infection, while PKC enhances the infection at the late stage.

机构信息

Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.

The Laboratory Animal Center, The Academic of Military Medical Sciences, Beijing 100071, P.R. China.

出版信息

Virus Res. 2017 Feb 2;229:41-47. doi: 10.1016/j.virusres.2016.12.010. Epub 2016 Dec 23.

DOI:10.1016/j.virusres.2016.12.010

PMID:28017735

Abstract

Porcine circovirus type 2 (PCV2) is the smallest DNA virus, which causes porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD). Due the small size of viral genomic DNA, PCV2 replication predominantly relies on the host factors. In this study, effects of PKC and HMGCR on PCV2 infection were evaluated using real time PCR and western blot. We found that PKC and HMGCR participated in different stages of PCV2 infection. HMGCR works on the early stage of the infection to inhibit the virus infection, while PKC enhances the infection at the late stage. Furthermore, PKC enhances PCV2 replication by activating JNK1/2 and inactivating HMGCR via regulating phosphorylation of these two proteins, while HMGCR can suppress phosphorylation of JNK1/2. The results in the present study will provide new sights in the pathogenesis of PCV2 infection, as well as interactions between host factors during PCV2 infection.

摘要

猪圆环病毒 2 型（PCV2）是最小的 DNA 病毒，可引起猪圆环病毒病和猪圆环病毒相关疾病（PCVD/PCVAD）。由于病毒基因组 DNA 较小，PCV2 的复制主要依赖于宿主因素。在这项研究中，使用实时 PCR 和 Western blot 评估了 PKC 和 HMGCR 对 PCV2 感染的影响。我们发现 PKC 和 HMGCR 参与了 PCV2 感染的不同阶段。HMGCR 在感染的早期阶段起作用以抑制病毒感染，而 PKC 在后期增强感染。此外，PKC 通过激活 JNK1/2 并通过调节这两种蛋白的磷酸化来使 HMGCR 失活，从而增强 PCV2 的复制，而 HMGCR 可以抑制 JNK1/2 的磷酸化。本研究的结果将为 PCV2 感染的发病机制以及 PCV2 感染过程中宿主因素之间的相互作用提供新的视角。