Feng Chang, Feng Man, Jiao Ran, Liu Dongyi, Jin Yanwu, Zhao Xin, Xiao Ruixue
Department of Anesthesiology, The Second Hospital of Shandong University, 247 Bei Yuan Street, Jinan 250033, China.
Department of Pathology, Affiliated Hospital of Shandong Academy of Medical Sciences, Shandong Academy of Medical Sciences, 38 Wu Yingshan Road, Jinan 250031, China.
Eur J Pharmacol. 2017 Feb 5;796:110-114. doi: 10.1016/j.ejphar.2016.12.035. Epub 2016 Dec 22.
Dezocine has been generally utilized for pain therapy and auxiliary anesthesia. Although it has some advantages on the prevention of some anesthesia related complications, its effect on immune responses remains unclear. Our study investigated the effects of Dezocine on IL-10 and IL-12 secretion and lymphocytes activation by culturing dendritic cells (DCs), and revealed the underlying mechanism. Mononuclear cells were divided into negative control group (GN), positive control group (GP), experimental group (GD; GD, D, D). DCs morphological structure was performed by microscope and its phenotypes were evaluated by flow cytometry. IL-12 and IL-10 levels were determined by ELISA and lymphocyte proliferation capacity was performed by MTT assay. Results showed that typical morphological characters of DCs were observed in GP and GD. The positive cell percentages of CD83, HLA-DR, CD80, CD86 and CD40 in GD were lower than those in GP, but higher than the GN group (P<0.01). IL-12 level in GD was higher than in GP, however, IL-10 was opposite (P<0.01). The optical density in GD was lower than in GP (P<0.05). There were no dose-dependent relationships correlated with DCs phenotypes, IL-12 and IL-10 secretion and lymphocytes activation (P>0.05). Our conclusion was that Dezocine might play a role in immunity by regulating IL-12 and IL-10 secretion, and affecting lymphocyte activity in process of DCs maturation. Our findings reveal an unexpected immuno-regulatory function of Dezocine in DCs and provide an important insight for investigating the effect of opioid drugs in immunologic responses.
地佐辛通常用于疼痛治疗和辅助麻醉。尽管它在预防一些与麻醉相关的并发症方面有一些优势，但其对免疫反应的影响仍不清楚。我们的研究通过培养树突状细胞（DCs）来研究地佐辛对白细胞介素-10（IL-10）和白细胞介素-12（IL-12）分泌以及淋巴细胞活化的影响，并揭示其潜在机制。单核细胞分为阴性对照组（GN）、阳性对照组（GP）、实验组（GD；GD、D、D）。通过显微镜观察DCs的形态结构，并通过流式细胞术评估其表型。采用酶联免疫吸附测定（ELISA）法测定IL-12和IL-10水平，采用噻唑蓝（MTT）比色法检测淋巴细胞增殖能力。结果显示，在GP组和GD组中观察到DCs的典型形态特征。GD组中CD83、HLA-DR、CD80、CD86和CD40的阳性细胞百分比低于GP组，但高于GN组（P<0.01）。GD组中IL-12水平高于GP组，然而，IL-10水平则相反（P<0.01）。GD组的光密度低于GP组（P<0.05）。DCs表型、IL-12和IL-10分泌以及淋巴细胞活化之间不存在剂量依赖关系（P>0.05）。我们的结论是，地佐辛可能通过调节IL-12和IL-10分泌以及影响DCs成熟过程中的淋巴细胞活性来发挥免疫作用。我们的研究结果揭示了地佐辛在DCs中意想不到的免疫调节功能，并为研究阿片类药物在免疫反应中的作用提供了重要的见解。
