Li Jinting, Wang Can, Han Xueping, Qi Wanzhen, Chen Yanqiong, Wang Taixia, Zheng Yi, Zhao Xiting
College of Life Sciences, Henan Normal UniversityXinxiang, China; Engineering Laboratory of Biotechnology for Green Medicinal Plant of Henan ProvinceXinxiang, China.
College of Life Sciences, Henan Normal University Xinxiang, China.
Front Plant Sci. 2016 Dec 12;7:1860. doi: 10.3389/fpls.2016.01860. eCollection 2016.
is a popular perennial medicine herb used for 1000s of years in China to treat various diseases. Although this herb has multiple pharmaceutical purposes in China, no transcriptomic information has been reported for this species. In addition, the understanding of several key pathways and enzymes involved in the biosynthesis of oleanolic acid and ecdysterone, two pharmacologically active classes of metabolites and major chemical constituents of root extracts, is limited. The aim of the present study was to characterize the transcriptome profile of the roots and leaves of to uncover the biosynthetic and transport mechanisms of the active components. In this study, we identified 100,987 transcripts, with an average length of 1146.8 base pairs. A total of 31,634 (31.33%) unigenes were annotated, and 12,762 unigenes were mapped to 303 pathways according to the Kyoto Encyclopedia of Genes and Genomes pathway database. Moreover, we identified a total of 260 oleanolic acid and ecdysterone genes encoding biosynthetic enzymes. Furthermore, the key enzymes involved in the oleanolic acid and ecdysterone synthesis pathways were analyzed using quantitative real-time polymerase chain reaction, revealing that the roots expressed these enzymes to a greater extent than the leaves. In addition, we identified 85 ATP-binding cassette transporters, some of which might be involved in the translocation of secondary metabolites.
是一种广受欢迎的多年生药草，在中国已有数千年用于治疗各种疾病的历史。尽管这种药草在中国有多种药用用途，但尚未有关于该物种的转录组信息报道。此外，对于齐墩果酸和蜕皮甾酮（两种具有药理活性的代谢产物类别以及根提取物的主要化学成分）生物合成过程中涉及的几个关键途径和酶的了解有限。本研究的目的是表征[药草名称未给出]根和叶的转录组图谱，以揭示活性成分的生物合成和转运机制。在本研究中，我们鉴定出100,987个转录本，平均长度为1146.8个碱基对。总共31,634个（31.33%）单基因被注释，并且根据京都基因与基因组百科全书途径数据库，12,762个单基因被映射到303条途径。此外，我们总共鉴定出260个编码生物合成酶的齐墩果酸和蜕皮甾酮基因。此外，使用定量实时聚合酶链反应分析了齐墩果酸和蜕皮甾酮合成途径中涉及的关键酶，结果表明根中这些酶的表达程度高于叶。此外，我们鉴定出85个ATP结合盒转运蛋白，其中一些可能参与次生代谢产物的转运。
