Bhattacharyya Paromik, Sharma Tanvi, Yadav Abhinandan, Lalthafamkimi Lucy, Swarnkar Mohit Kumar, Joshi Robin, Shankar Ravi, Kumar Sanjay
Biotechnology Division, Council of Scientific and Industrial Research-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India.
Studio of Computational Biology & Bioinformatics, The Himalayan Centre for High-throughput Computational Biology (HiCHiCoB, A BIC supported by DBT, India), Council for Scientific and Industrial Research (CSIR)-Institute of Himalayan Bioresource Technology (CSIR-IHBT), Palampur, Himachal Pradesh, India.
Front Plant Sci. 2022 Oct 18;13:954467. doi: 10.3389/fpls.2022.954467. eCollection 2022.
D. Don [= (D. Don) Szlach.] is an endangered medicinal orchid of the group of plants in (Indian system of traditional medicine). Using a combination of aromatic cytokinin [-Topolin (T)], plant biostimulant (chitosan), auxin [indole-3-butyric acid (IBA)], and a phenolic elicitor [phloroglucinol (PG)], plants of were regenerated for mass multiplication. The present research reveals the first-ever transcriptome of . A total of 43,111 transcripts encoding 23,951 unigenes were assembled from a total of 815.02 million reads obtained from leaf and pseudobulb of raised Expression analysis of genes associated with β-sitosterol and eugenol biosynthesis in leaf and pseudobulb provided vital clues for differential accumulation of metabolites in . Ultra-performance liquid chromatography (UPLC) confirmed higher amounts of β-sitosterol and eugenol content in the leaf as compared to the pseudobulb. Differential expression of transcripts related to starch and sucrose metabolism, plant hormone signal transduction, diterpenoid biosynthesis, phenylalanine metabolism, stilbenoid, diarylheptanoid, and gingerol biosynthesis suggested the operation of differential metabolic pathways in leaf and pseudobulb. The present research provides valuable information on the biosynthesis of secondary metabolites in , which could be used for advanced metabolite bioprospection using cell suspension culture and bioreactor-based approaches. Data also suggested that leaf tissues rather than pseudobulb can be used as an alternate source of bioactive metabolites thereby shifting the need for harvesting the pseudobulb. This will further facilitate the conservation and sustainable utilization of this highly valued medicinal orchid.
D. Don [= (D. Don) Szlach.] 是（印度传统医学体系）中一组植物里的一种濒危药用兰花。通过将芳香细胞分裂素 [- 托布津 (T)]、植物生物刺激素（壳聚糖）、生长素 [吲哚 - 3 - 丁酸 (IBA)] 和一种酚类诱导剂 [间苯三酚 (PG)] 相结合，实现了D. Don植株的再生以进行大规模繁殖。本研究揭示了D. Don有史以来的首个转录组。从D. Don的叶片和假鳞茎获得的总共8.1502亿条 reads 中组装出了总共43,111条转录本，编码23,951个单基因。对叶片和假鳞茎中与β - 谷甾醇和丁香酚生物合成相关基因的表达分析为D. Don中代谢物的差异积累提供了重要线索。超高效液相色谱 (UPLC) 证实，与假鳞茎相比，叶片中β - 谷甾醇和丁香酚的含量更高。与淀粉和蔗糖代谢、植物激素信号转导、二萜生物合成、苯丙氨酸代谢、芪类、二芳基庚烷类和姜辣素生物合成相关的转录本的差异表达表明叶片和假鳞茎中存在不同的代谢途径。本研究提供了关于D. Don中次生代谢物生物合成的有价值信息，可用于使用细胞悬浮培养和基于生物反应器的方法进行高级代谢物生物勘探。数据还表明，叶片组织而非假鳞茎可作为生物活性代谢物的替代来源，从而改变了对收获假鳞茎的需求。这将进一步促进这种高价值药用兰花的保护和可持续利用。
