Naz Iffat, Hodgson Douglas, Smith Ann, Marchesi Julian, Ahmed Safia, Avignone-Rossa Claudio, Saroj Devendra P
Department of Civil and Environmental Engineering, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford GU2 7XH, UK. Email:
Department of Microbial Sciences, University of Surrey, Guildford GU2 7XH, UK.
RSC Adv. 2016 Nov 1;6(106):104345-104353. doi: 10.1039/c6ra21040f. Epub 2016 Oct 26.
This study investigates the microbial community composition in the biofilms grown on two different support media in fixed biofilm reactors for aerobic wastewater treatment, using next generation sequencing (NGS) technology. The chemical composition of the new type of support medium (TDR) was found to be quite different from the conventionally used support medium (stone). The analysis of 16S rRNA gene fragments recovered from the laboratory scale biofilm system show that biofilm support media and temperature conditions influence bacterial community structure and composition. Greater bacterial diversity was observed under each condition, primarily due to the large number of sequences available and sustenance of rare species. There were 6 phyla found, with the highest relative abundance shown by the phylum (52.71%) followed by (33.33%), (4.65%), , (3.1%) and (>1%). The dataset showed 17 genera of bacterial populations to be commonly shared under all conditions, suggesting the presence of a core microbial community in the biofilms for wastewater treatment. However, some genera in the biofilms on TDR were observed in high proportions, which may be attributed to its chemical composition, explaining the improved level of wastewater treatment. The findings show that the structure of microbial communities in biofilm systems for wastewater treatment is affected by the properties of support matrix.
本研究采用下一代测序（NGS）技术，调查了用于好氧废水处理的固定生物膜反应器中，在两种不同载体介质上生长的生物膜中的微生物群落组成。发现新型载体介质（TDR）的化学成分与传统使用的载体介质（石头）有很大不同。从实验室规模的生物膜系统中回收的16S rRNA基因片段分析表明，生物膜载体介质和温度条件会影响细菌群落结构和组成。在每种条件下都观察到了更高的细菌多样性，这主要是由于可用序列数量众多以及稀有物种得以维持。共发现6个门，相对丰度最高的是 门（52.71%），其次是 门（33.33%）、 门（4.65%）、 门、 门（3.1%）和 门（>1%）。数据集显示，在所有条件下共有17个细菌种群属，这表明在用于废水处理的生物膜中存在一个核心微生物群落。然而，在TDR上的生物膜中，一些属的比例很高，这可能归因于其化学成分，解释了废水处理水平的提高。研究结果表明，用于废水处理的生物膜系统中微生物群落的结构受载体基质特性的影响。
