Mohammed Amal E, Behiry Eman G, El-Sadek Akram E, Abdulghany Waleed E, Mahmoud Dalia M, Elkholy Abdelfattah A
Clinical & Chemical Pathology Department, Faculty of Medicine, Benha University, Egypt.
Pediatric Department, Faculty of Medicine, Benha University, Egypt.
Ann Med Surg (Lond). 2016 Dec 1;13:6-12. doi: 10.1016/j.amsu.2016.11.046. eCollection 2017 Jan.
This study aims to investigate the association of neonatal indirect hyperbilirubinemia in exclusively breast-fed infants with UGT1A1 (Uridine Diphosphate-Glucuronyl transferase 1A1) polymorphism.
50 neonates were classified into 2 groups: 1) 30 full term neonates with indirect hyperbilirubinemia (gestational age (GA) 39.5 ± 1.2 weeks); 2) 20 apparently healthy full-term neonates. Group 1 was further subdivided based on percentage of body weight lost: (A) less than 10%; (B) 10% or more.
There was a statistically significant decrease in weight at sample collection and significant increase in indirect bilirubin level in patients group compared to control group, there was statistically significant difference as regard to genotype frequency [G/G, G/A, A/A], and allele frequency (A,G) between patients and control group. There was statistically significant increase in indirect bilirubin level in G/A - A/A genotypes. By comparing subgroup (A) and subgroup (B), there was statistically significant increase in total bilirubin level in subgroup (B). There was statistically high significant difference regarding genotype frequency (G/G, G/A, A/A) and allele frequency (G, A) between subgroup A and B. Multiple stepwise regression analysis was done using hyperbilirubinemia as a dependent factor and body weight loss, genotype (G/A) and allele (A) as independent factors. Body weight loss, genotype (G/A) and allele (A) was found to be significant independent predictors for hyperbilirubinemia.
The results of the present study revealed that UGT1A1 polymorphism can be used as a novel predictor for neonatal hyperbilirubinemia in breast fed full term neonates.
本研究旨在探讨纯母乳喂养婴儿的新生儿间接高胆红素血症与尿苷二磷酸葡萄糖醛酸转移酶1A1（UGT1A1）基因多态性之间的关联。
50例新生儿分为2组：1）30例足月间接高胆红素血症新生儿（胎龄（GA）39.5±1.2周）；2）20例明显健康的足月新生儿。第1组根据体重减轻百分比进一步细分：（A）小于10%；（B）10%或更多。
与对照组相比，患者组样本采集时体重有统计学意义的下降，间接胆红素水平有显著升高，患者组与对照组在基因型频率[G/G、G/A、A/A]和等位基因频率（A、G）方面存在统计学显著差异。G/A - A/A基因型的间接胆红素水平有统计学意义的升高。通过比较亚组（A）和亚组（B），亚组（B）的总胆红素水平有统计学意义的升高。亚组A和B在基因型频率（G/G、G/A、A/A）和等位基因频率（G、A）方面存在统计学高度显著差异。以高胆红素血症为因变量，体重减轻、基因型（G/A）和等位基因（A）为自变量进行多元逐步回归分析。发现体重减轻、基因型（G/A）和等位基因（A）是高胆红素血症的重要独立预测因素。
本研究结果表明，UGT1A1基因多态性可作为足月母乳喂养新生儿高胆红素血症的一种新的预测指标。
