da Silva Gabriela T, Voss Guilherme T, Kaplum Vanessa, Nakamura Celso V, Wilhelm Ethel A, Luchese Cristiane, Fajardo André R
Laboratório de Tecnologia e Desenvolvimento de Materiais Poliméricos e Compósitos - LaCoPol. Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas (UFPel), 96010-900 Pelotas, RS, Brazil.
Grupo de Pesquisa em Neurobiotecnologia (GPN). Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas (UFPel), 96010-900 Pelotas, RS, Brazil.
Mater Sci Eng C Mater Biol Appl. 2017 Mar 1;72:526-535. doi: 10.1016/j.msec.2016.11.069. Epub 2016 Nov 22.
Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions. The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability. At 10wt.% BBP the biocomposite exhibited enhanced mechanical properties and biodegradability rate as compared to the pristine sample. Further, different properties of the biocomposite can be tailored according to the content of BBP. In vitro assays showed that ChS/PVA-BBP does not exert cytotoxicity against healthy cells. In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses. The biocomposite was completely biodegraded and bioresorbed after 15days of treatment. Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance. All these results suggest that the biocomposite designed in this study is a promising biomaterial for potential skin tissue engineering.
硫酸软骨素（ChS），一种硫酸化糖胺聚糖，与聚乙烯醇（PVA）和牛骨粉（BBP）在温和条件下通过循环冻融混合形成一种新型环保生物复合材料。系统研究表明，BBP的含量对孔径、孔隙率、力学性能、液体吸收性能和生物降解性有显著影响。与原始样品相比，在BBP含量为10wt.%时，生物复合材料表现出增强的力学性能和生物降解率。此外，生物复合材料的不同性能可根据BBP的含量进行调整。体外试验表明，ChS/PVA-BBP对健康细胞无细胞毒性。体内和体外实验表明，ChS/PVA-BBP生物复合材料是生物相容性材料，不会引发促炎反应。治疗15天后，生物复合材料完全生物降解并被生物吸收。综上所述，BBP是低成本的羟基磷灰石和胶原蛋白来源，这是有保障的。所有这些结果表明，本研究设计的生物复合材料是一种有前途的潜在皮肤组织工程生物材料。
