Lee Na Eun, Kim Sung Joo, Yang Seung-Jib, Joo Sung-Yeon, Park Hyojun, Lee Kyo Won, Yang Heung-Mo, Park Jae Berm
Transplantation Research Center, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Republic of Korea; Samsung Advanced Institute for Health Sciences & Technology, Graduate School, Department of Health Sciences & Technology, Sungkyunkwan University, Kyunggi, Republic of Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea.
Transplantation Research Center, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Republic of Korea; Samsung Advanced Institute for Health Sciences & Technology, Graduate School, Department of Health Sciences & Technology, Sungkyunkwan University, Kyunggi, Republic of Korea; Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul, Republic of Korea; Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
Cytotherapy. 2017 Feb;19(2):170-180. doi: 10.1016/j.jcyt.2016.11.002. Epub 2016 Dec 23.
There are various types of adipose tissue in the human body, and their morphology is known to be closely related to cell function and metabolism. However, the functional differences among the mesenchymal stromal cells (MSCs) of different abdominal adipose tissues have not been clearly elucidated.
MSCs were isolated from different abdominal adipose tissues according to their regional distribution and included superficial subcutaneous, deep subcutaneous, omentum, mesentery and retroperitoneal MSCs. The immunophenotype, proliferative ability and angiogenic function of these MSCs were compared based on flow cytometry analysis, CCK-8 proliferation, in vitro differentiation, tubule formation and in vivo plug assay.
The plastic adherence, cell morphology and general immunophenotype are similar among the MSCs. However, subcutaneous adipose tissue-derived MSCs have a faster growth rate and a higher level of CD146 expression than the other MSCs. Moreover, according to the fluorescence-activated cell sorting (FACS) enrichment procedure, the expression level of CD146 is positively related to the growth rate and angiogenic capability of MSCs.
MSCs in adipose tissue showed slightly different characteristics depending on their location of origin, and they possessed different angiogenic abilities that were mediated by the expression of CD146. This study provides evidence that subcutaneous adipose tissue is the most appropriate source of MSCs for therapeutic cell transplantation in vascular disease.
人体存在多种类型的脂肪组织，已知其形态与细胞功能和代谢密切相关。然而，不同腹部脂肪组织间的间充质基质细胞（MSC）的功能差异尚未明确阐明。
根据区域分布从不同腹部脂肪组织中分离出MSC，包括浅表皮下、深部皮下、网膜、肠系膜和腹膜后MSC。基于流式细胞术分析、CCK-8增殖、体外分化、小管形成和体内植入试验，比较这些MSC的免疫表型、增殖能力和血管生成功能。
MSC之间的贴壁生长、细胞形态和一般免疫表型相似。然而，皮下脂肪组织来源的MSC比其他MSC具有更快的生长速度和更高水平的CD146表达。此外，根据荧光激活细胞分选（FACS）富集程序，CD146的表达水平与MSC的生长速度和血管生成能力呈正相关。
脂肪组织中的MSC根据其起源位置表现出略有不同的特征，并且它们具有由CD146表达介导的不同血管生成能力。本研究提供了证据，表明皮下脂肪组织是血管疾病治疗性细胞移植中最合适的MSC来源。
