University of Wisconsin School of Medicine and Public Health, Madison, WI 53792, USA.
Aesthet Surg J. 2013 Aug 1;33(6):888-98. doi: 10.1177/1090820X13496115.
Adipose-derived mesenchymal stem cells (ADSC) may have a potential dual role in soft tissue augmentation by suppressing inflammation and promoting regeneration. Due to these properties, there is increasing interest in their potential use in autologous fat grafting, particularly to the breast.
The authors isolate and compare ADSC derived from abdominal and breast tissues with a hypothesis that different adipose tissue sources may demonstrate different functional characteristics affecting outcomes in autologous cell transplantation in reconstructive and aesthetic surgery.
Adipose-derived mesenchymal stem cells from abdominal and breast tissues were isolated and compared in terms of surface marker expression, differentiation capabilities, and both fibroblast growth factor (FGF) and receptor expression. Immunophenotype of macrophages was also investigated using cell surface markers following a 7-day co-culture period with ADSC.
Results showed similar cell surface phenotype and multilineage differentiation capabilities of ADSC derived from abdominal and breast tissues. Variations of FGF expression were demonstrated on reverse transcription polymerase chain reaction, with a significantly higher expression of FGF2 seen in breast ADSC. Following the 7-day co-culture period, increased expression of the anti-inflammatory surface marker CD206 was identified, with decreased CD16 and human leukocyte antigen-DR on macrophages co-cultured with ADSC compared with controls.
The data indicate similarities between ADSC derived from abdominal and breast tissues. Significant differences were seen, however, in the expression of FGF2, which is important in angiogenesis and wound healing. The results support the utility of ADSC in cell-based therapies such as autologous fat grafting.
脂肪来源的间充质干细胞（ADSC）通过抑制炎症和促进再生，可能在软组织增强中具有潜在的双重作用。由于这些特性，人们对其在自体脂肪移植中的潜在用途越来越感兴趣，特别是在乳房。
作者分离并比较了来自腹部和乳房组织的 ADSC，并提出了一个假设，即不同的脂肪组织来源可能表现出不同的功能特征，影响重建和美容手术中自体细胞移植的结果。
从腹部和乳房组织中分离和比较 ADSC，从表面标志物表达、分化能力以及成纤维细胞生长因子（FGF）及其受体表达等方面进行比较。还通过与 ADSC 共培养 7 天后使用细胞表面标志物研究巨噬细胞的免疫表型。
结果表明，来自腹部和乳房组织的 ADSC 具有相似的细胞表面表型和多能分化能力。逆转录聚合酶链反应显示 FGF 表达存在差异，乳腺 ADSC 中 FGF2 的表达明显更高。经过 7 天共培养期后，发现抗炎表面标志物 CD206 的表达增加，与对照组相比，与 ADSC 共培养的巨噬细胞 CD16 和人类白细胞抗原-DR 减少。
数据表明，来自腹部和乳房组织的 ADSC 之间存在相似性。然而，FGF2 的表达存在显著差异，FGF2 对血管生成和伤口愈合很重要。结果支持 ADSC 在细胞治疗中的应用，如自体脂肪移植。
