大肠杆菌中FLP重组酶的严格控制。

Stringent control of FLP recombinase in Escherichia coli.

作者信息

Bowden Steven D, Palani Nagendra P, Libourel Igor G L

机构信息

Biotechnology institute, 1500 Gortner Avenue, University of Minnesota, Saint Paul, MN 55108, United States.

Department of Plant Biology, University of Minnesota, Saint Paul, MN 55108, United States.

出版信息

J Microbiol Methods. 2017 Feb;133:52-54. doi: 10.1016/j.mimet.2016.12.018. Epub 2016 Dec 24.

DOI:10.1016/j.mimet.2016.12.018

PMID:28024983

Abstract

Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.

摘要

位点特异性重组酶是分子生物学中非常有价值的工具，并且正在成为合成生物学中细胞事件的强大记录器。我们利用阿拉伯糖诱导型启动子与弱核糖体结合位点相结合，在大肠杆菌中开发了一种严格控制的FLP重组酶系统。