Sawicki J A, Monks B, Morris R J
Lankenau Medical Research Center, Wynnewood, PA, USA.
Biotechniques. 1998 Nov;25(5):868-70, 872-5. doi: 10.2144/98255rr01.
The ability of site-specific recombinases, like FLP and Cre, to catalyze alterations in genomic DNA is well established, whereas their application to genetic engineering strategies has been restricted because of the inability to temporally regulate their expression and subsequent recombination events in specific populations of cells. We describe a regulatory system for ecdysone-controlled expression of FLP recombinase. Furthermore, we demonstrate that ecdysone-induced, FLP-mediated site-specific recombination events can be targeted to specific cells. This system can be applied to cell-lineage studies as well as to the design of gene-therapy strategies, particularly in stem cells.
位点特异性重组酶，如FLP和Cre，催化基因组DNA改变的能力已得到充分证实，然而由于无法在特定细胞群体中对其表达及随后的重组事件进行时间调控，它们在基因工程策略中的应用受到了限制。我们描述了一种用于蜕皮激素控制FLP重组酶表达的调控系统。此外，我们证明蜕皮激素诱导的、FLP介导的位点特异性重组事件能够靶向特定细胞。该系统可应用于细胞谱系研究以及基因治疗策略的设计，尤其是在干细胞中。
