Koshida Ryusuke, Oishi Hisashi, Hamada Michito, Takei Yosuke, Takahashi Satoru
Department of Anatomy and Neuroscience, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan.
Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan.
Biochem Biophys Res Commun. 2017 Jan 29;483(1):288-293. doi: 10.1016/j.bbrc.2016.12.150. Epub 2016 Dec 23.
The choroid plexus (ChP) is a non-neural epithelial tissue that produces cerebrospinal fluid (CSF). The ChP differentiates from the roof plate, a dorsal midline structure of the neural tube. However, molecular mechanisms underlying ChP development are poorly understood compared to neural development. MafB is a bZip transcription factor that is known to be expressed in the roof plate. Here we investigated the role of MafB in embryonic development of the hindbrain ChP (hChP) using Mafb-deficient mice. Immunohistochemical analyses revealed that MafB is expressed in the roof plate and early hChP epithelial cells but its expression disappears at a later embryonic stage. We also found that the Mafb-deficient hChP exhibits delayed differentiation and results in hypoplasia compared to the wild-type hChP. Furthermore, the Mafb-deficient hChP exhibits increased apoptotic cell death and decreased proliferating cells at E12.5, an early stage of hChP development. Collectively, our findings reveal that MafB play an important role in promoting hChP development during embryogenesis.
脉络丛（ChP）是一种产生脑脊液（CSF）的非神经上皮组织。脉络丛由神经管的背侧中线结构顶板分化而来。然而，与神经发育相比，脉络丛发育的分子机制尚不清楚。MafB是一种bZip转录因子，已知在顶板中表达。在这里，我们使用Mafb基因缺陷小鼠研究了MafB在后脑脉络丛（hChP）胚胎发育中的作用。免疫组织化学分析显示，MafB在顶板和早期hChP上皮细胞中表达，但其表达在胚胎后期消失。我们还发现，与野生型hChP相比，Mafb基因缺陷的hChP表现出分化延迟并导致发育不全。此外，在hChP发育的早期阶段E12.5，Mafb基因缺陷的hChP表现出凋亡细胞死亡增加和增殖细胞减少。总的来说，我们的研究结果表明，MafB在胚胎发育过程中促进hChP发育方面发挥着重要作用。
