Ma Yingchang, Zhang Yang, Zhai Yuanjuan, Zhu Zhenhua, Pan Ying, Qian Dawei, Su Shulan, Fan Xinsheng, Duan Jinao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Molecules. 2016 Dec 22;22(1):7. doi: 10.3390/molecules22010007.
Haizao Yuhu Decoction (HYD) has been used for approximately 500 years and is well-known in Traditional Chinese Medicine for its efficacy in the treatment of thyroid-related diseases. In this study, a rapid liquid chromatography-tandem mass spectrometry method was developed for the determination of liquiritin, naringin, hesperidin, peimine, liquiritigenin, glycyrrhizic acid, bergapten, nobiletin, osthole, and glycyrrhetinic acid in rat plasma to investigate the pharmacokinetic profile of different HYD prescriptions in a rat model of hypothyroidism. The differences in pharmacokinetic parameters among the groups were compared by Student's -test. The pharmacokinetic profile of liquiritin, naringin, hesperidin, peimine, liquiritigenin, glycyrrhizic acid, bergapten, nobiletin, osthole, and glycyrrhetinic acid showed significant differences between Haizao and Gancao anti-drug combination and other herbs in HYD. These results may contribute to the rational clinical use of HYD and reveal the compatibility profile of the Haizao and Gancao anti-drug combination.
海藻玉壶汤（HYD）已应用约500年，在中医领域因其治疗甲状腺相关疾病的疗效而闻名。在本研究中，建立了一种快速液相色谱 - 串联质谱法，用于测定大鼠血浆中的甘草苷、柚皮苷、橙皮苷、浙贝母碱、甘草素、甘草酸、补骨脂素、川陈皮素、蛇床子素和甘草次酸，以研究不同海藻玉壶汤方剂在甲状腺功能减退大鼠模型中的药代动力学特征。通过学生t检验比较各组药代动力学参数的差异。甘草苷、柚皮苷、橙皮苷、浙贝母碱、甘草素、甘草酸、补骨脂素、川陈皮素、蛇床子素和甘草次酸的药代动力学特征在海藻与甘草反药组合及海藻玉壶汤中的其他草药之间存在显著差异。这些结果可能有助于海藻玉壶汤的合理临床应用，并揭示海藻与甘草反药组合的配伍特征。
