Lambirth Kevin C, Whaley Adam M, Schlueter Jessica A, Piller Kenneth J, Bost Kenneth L
Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, North Carolina Research Campus, Kannapolis, NC 28081, USA.
Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.
Int J Plant Genomics. 2016;2016:1562041. doi: 10.1155/2016/1562041. Epub 2016 Nov 29.
Transgenic crops have been utilized for decades to enhance agriculture and more recently have been applied as bioreactors for manufacturing pharmaceuticals. Recently, we investigated the gene expression profiles of several in-house transgenic soybean events, finding one transformant group to be consistently different from our controls. In the present study, we examined polymorphisms and sequence variations in the exomes of the same transgenic soybean events. We found that the previously dissimilar soybean line also exhibited markedly increased levels of polymorphisms within mRNA transcripts from seed tissue, many of which are classified as gene expression modifiers. The results from this work will direct future investigations to examine novel SNPs controlling traits of great interest for breeding and improving transgenic soybean crops. Further, this study marks the first work to investigate SNP rates in transgenic soybean seed tissues and demonstrates that while transgenesis may induce abundant unanticipated changes in gene expression and nucleotide variation, phenotypes and overall health of the plants examined remained unaltered.
转基因作物已被利用数十年以促进农业发展，最近还被用作生产药物的生物反应器。最近，我们研究了几个内部转基因大豆事件的基因表达谱，发现一个转化体组与我们的对照始终不同。在本研究中，我们检查了相同转基因大豆事件外显子组中的多态性和序列变异。我们发现，之前表现不同的大豆品系在种子组织的mRNA转录本中也表现出多态性水平显著增加，其中许多被归类为基因表达调节剂。这项工作的结果将指导未来的研究，以检查控制对育种和改良转基因大豆作物具有重大意义的性状的新型单核苷酸多态性（SNP）。此外，本研究标志着首次对转基因大豆种子组织中的SNP率进行研究，并表明虽然转基因可能会在基因表达和核苷酸变异中引发大量意外变化，但所检查植物的表型和整体健康状况并未改变。
