Hultberg Malin, Lind Olle, Birgersson Göran, Asp Håkan
Department of Biosystems and Technology, Swedish University of Agricultural Sciences, P.O. Box 103, 230 53, Alnarp, Sweden.
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Alnarp, Sweden.
Bioprocess Biosyst Eng. 2017 Apr;40(4):625-631. doi: 10.1007/s00449-016-1726-2. Epub 2016 Dec 26.
The effluent from the biogas process was tested as a nutrient source during cultivation of the protein-rich and edible microalgae Spirulina (Arthrospira platensis) and compared with conventional Spirulina medium. Equal biomass production was observed until late exponential phase and no significant differences could be observed between the treatments in protein amount, amino acid composition, and total lipid concentration. The concentration of the pigment phycocyanin differed significantly between Spirulina medium and the effluent-based medium (63.3 ± 11.7 and 86.2 ± 1.9 mg g, respectively). Slightly higher concentrations of saturated fatty acids, mainly palmitic acid, were observed in the biomass produced in Spirulina medium than in that produced in the effluent-based medium. In the biomass produced in the effluent-based medium, the cadmium concentration was 0.07 ± 0.05 mg kg of dry weight, whereas it was below the detection limit in the biomass produced in Spirulina medium. There is a need to identify new food and feed resources and a possible future scenario is to integrate Spirulina production into the biogas plant for protein production as it contains more than 60% of protein on dry weight basis. In that scenario, it is important to control heavy metal concentrations in the biogas slurry fed to Spirulina.
在富含蛋白质且可食用的微藻螺旋藻（钝顶节旋藻）培养过程中，对沼气工艺产生的废水作为营养源进行了测试，并与传统的螺旋藻培养基进行了比较。在指数生长后期之前，观察到生物量产量相当，且在蛋白质含量、氨基酸组成和总脂质浓度方面，各处理之间未观察到显著差异。螺旋藻培养基和基于废水的培养基中藻蓝蛋白色素的浓度存在显著差异（分别为63.3±11.7和86.2±1.9毫克/克）。在螺旋藻培养基中产生的生物量中观察到的饱和脂肪酸浓度略高于基于废水的培养基中产生的生物量，主要是棕榈酸。在基于废水的培养基中产生的生物量中，镉浓度为0.07±0.05毫克/千克干重，而在螺旋藻培养基中产生的生物量中镉浓度低于检测限。有必要确定新的食物和饲料资源，未来一种可能的情况是将螺旋藻生产整合到沼气厂中以生产蛋白质，因为其干重基础上含有超过60%的蛋白质。在这种情况下，控制供给螺旋藻的沼液中的重金属浓度很重要。
