Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China.
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China.
Bioresour Technol. 2018 Mar;252:14-19. doi: 10.1016/j.biortech.2017.12.046. Epub 2017 Dec 18.
To improve the microalgae production in batch cultivation, a cultivation mode that continuously pre-harvesting Spirulina platensis from photobioreactor (PBR) with culture medium recycling was proposed. For realizing the continuously pre-harvesting cultivation mode, a Spirulina platensis culture column PBR with overflowing device was designed, which could adjust pre-harvesting rate through the overflowing device. By adjusting the pre-harvesting rate, the biomass concentration could be kept when biomass accumulation and pre-harvesting biomass were equal. Hence, the meridional light attenuation could be reduced by controlling biomass concentration in PBR. The maximum microalgae production were 44.6%, 10.98% higher in total production than that cultivated in batch cultivation without pre-harvesting and periodically pre-harvesting cultivation mode respectively, which was realized in pre-harvesting rate 0.228 mL min and biomass concentration 1.8 g L. Besides, a model was built by mass balance and polynomial fitting for evaluating the continuously pre-harvesting cultivation mode.
为提高批量培养中的微藻产量，提出了一种连续从光生物反应器（PBR）中用培养液循环收获螺旋藻的培养模式。为实现连续收获培养模式，设计了一种带溢流装置的螺旋藻培养柱 PBR，可通过溢流装置调节收获率。通过调节收获率，当生物量积累和收获生物量相等时，可以保持生物量浓度。因此，通过控制 PBR 中的生物量浓度，可以减少南北向光衰减。最大微藻产量分别比不收获和定期收获培养模式下的批量培养提高了 44.6%和 10.98%，收获率为 0.228 mL min，生物量浓度为 1.8 g L。此外，还通过质量平衡和多项式拟合建立了一个模型，用于评估连续收获培养模式。
