Makrinos D L, Bowden T J
School of Food and Agriculture, University of Maine, Orono, ME, USA.
J Fish Dis. 2017 Aug;40(8):1115-1127. doi: 10.1111/jfd.12578. Epub 2016 Dec 27.
Piscirickettsia salmonis is an intracellular bacterium that was first isolated and identified in fish cells. Several types of cell lines have been explored for their ability to provide the bacterium with a host cell to replicate in. Tissue culture has been used for growth and cultivation for nearly two decades, until the facultative nature of P. salmonis was confirmed upon the development of blood- and cysteine-based agar. Since then, research has continued to drive the creation of novel agar and broth formulations in order to improve the efficacy of cultivation of P. salmonis. Until now, the techniques and components used for growth have not been thoroughly discussed. In this review, the methods and formulations for growth of P. salmonis in tissue culture and cell-free media will be examined.
鲑鱼立克次氏体是一种胞内细菌，最初是在鱼类细胞中分离和鉴定出来的。人们已经探索了几种细胞系为该细菌提供复制宿主细胞的能力。组织培养已用于其生长和培养近二十年，直到基于血液和半胱氨酸的琼脂出现后，鲑鱼立克次氏体的兼性才得以证实。从那时起，研究不断推动新型琼脂和肉汤配方的开发，以提高鲑鱼立克次氏体的培养效果。到目前为止，用于生长的技术和成分尚未得到充分讨论。在这篇综述中，将研究鲑鱼立克次氏体在组织培养和无细胞培养基中的生长方法和配方。
