Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
PLoS One. 2013 Sep 5;8(9):e71830. doi: 10.1371/journal.pone.0071830. eCollection 2013.
Piscirickettsia salmonis is the bacterium that causes Piscirickettsiosis, a systemic disease of salmonid fish responsible for significant economic losses within the aquaculture industry worldwide. The growth of the bacterium for vaccine formulation has been traditionally accomplished by infecting eukaryotic cell lines, a process that involves high production costs and is time-consuming. Recent research has demonstrated that it is possible to culture pure P. salmonis in a blood containing (cell-free) medium. In the present work we demonstrate the growth of P. salmonis in a liquid medium free from blood and serum components, thus establishing a novel and simplified bacteriological medium. Additionally, the new media reported provides improved growth conditions for P. salmonis, where biomass concentrations of approximately 800 mg cell dry weight L(-1) were obtained, about eight times higher than those reported for the blood containing medium. A 2- level full factorial design was employed to evaluate the significance of the main medium components on cell growth and an optimal temperature range of 23-27°C was determined for the microorganism to grow in the novel liquid media. Therefore, these results represent a breakthrough regarding P. salmonis research in order to optimize pure P. salmonis growth in liquid blood and serum free medium.
鲑鱼鱼立克次氏体是引起鱼立克次体病的细菌，这种全身性疾病会给世界范围内的水产养殖业造成重大经济损失。传统上，为了疫苗的配方，细菌的生长是通过感染真核细胞系来完成的，这个过程涉及到高昂的生产成本和耗时。最近的研究表明，在含有（无细胞）血液的培养基中培养纯鲑鱼鱼立克次体是可行的。在本工作中，我们证明了在无血液和血清成分的液体培养基中鲑鱼鱼立克次体的生长，从而建立了一种新型的简化细菌培养基。此外，所报道的新培养基为鲑鱼鱼立克次体提供了更好的生长条件，获得了约 800mg 细胞干重/L 的生物量浓度，比含血液培养基报道的生物量浓度高约 8 倍。采用 2 水平完全析因设计来评估主要培养基成分对细胞生长的重要性，并确定了该微生物在新型液体培养基中生长的最佳温度范围为 23-27°C。因此，这些结果代表了鲑鱼鱼立克次体研究的一个突破，以便优化在无血液和血清的液体培养基中纯鲑鱼鱼立克次体的生长。
