Gil-Sánchez Irene, Ayuda-Durán Begoña, González-Manzano Susana, Santos-Buelga Celestino, Cueva Carolina, Martín-Cabrejas M Angeles, Sanz-Buenhombre Marisa, Guadarrama Alberto, Moreno-Arribas M Victoria, Bartolomé Begoña
Institute of Food Science Research (CIAL), CSIC-UAM, Madrid, Spain.
Grupo de Investigación en Polifenoles, Universidad de Salamanca, Salamanca, Spain.
J Sci Food Agric. 2017 Aug;97(10):3433-3444. doi: 10.1002/jsfa.8197. Epub 2017 Jan 26.
Currently, there is growing interest in extracts derived from winery by-products because of their beneficial health properties, which are associated with the presence of bioactive compounds. In this paper, we have carried out the chemical characterization and in vitro colonic fermentation of four grape pomace (GP) extracts rich in polyphenols and dietary fibre.
Firstly, phenolic and dietary fibre composition of the GP extracts was determined. The highest individual phenolic concentrations corresponded to gallic and ellagic acids, followed by catechins and flavonols. The non-digestible fibre fraction ranged from 66% to 83% of the GP extracts, which indicated that they mainly contained non-digestible cell wall components. Secondly, when GP extracts were subjected to fermentation by faecal microbiota, a total of 16 bacterial phenolic metabolites were found in the fermented samples, confirming that polyphenols contained in the GP extracts were metabolized to different active metabolites by microbiota. In addition, the GP extracts tended to promote the growth of intestinal microbiota, although it was only significant for the Enterococcus group.
These findings, together with other information available in the literature, support the high added value of products obtained from winery by-products. © 2016 Society of Chemical Industry.
目前，由于葡萄酒厂副产品提取物具有有益健康的特性，且与生物活性化合物的存在有关，人们对其兴趣日益浓厚。在本文中，我们对四种富含多酚和膳食纤维的葡萄皮渣（GP）提取物进行了化学表征和体外结肠发酵研究。
首先，测定了GP提取物的酚类和膳食纤维组成。酚类物质的最高个体浓度对应于没食子酸和鞣花酸，其次是儿茶素和黄酮醇。不可消化纤维部分占GP提取物的66%至83%，这表明它们主要含有不可消化的细胞壁成分。其次，当GP提取物经粪便微生物群发酵时，在发酵样品中总共发现了16种细菌酚类代谢物，证实了GP提取物中含有的多酚被微生物群代谢为不同的活性代谢物。此外，GP提取物倾向于促进肠道微生物群的生长，尽管仅对肠球菌属有显著影响。
这些发现与文献中的其他信息一起，支持了从葡萄酒厂副产品中获得的产品具有高附加值。©2016化学工业协会。
