Bi Fangcheng, Ment Dana, Luria Neta, Meng Xiangchun, Prusky Dov
Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou 510640, China; Key Laboratory of Tropical and Subtropical Fruit Tree Research, Guangdong Province, Guangzhou 510640, China; Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel.
Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel.
Fungal Genet Biol. 2017 Feb;99:29-39. doi: 10.1016/j.fgb.2016.12.006. Epub 2016 Dec 25.
The GATA transcription factor AreA is a global nitrogen regulator that restricts the utilization of complex and poor nitrogen sources in the presence of good nitrogen sources in microorganisms. In this study, we report the biological function of an AreA homolog (the CgareA gene) in the fruit postharvest pathogen Colletotrichum gloeosporioides. Targeted gene deletion mutants of areA exhibited significant reductions in vegetative growth, increases in conidia production, and slight decreases in conidial germination rates. Quantitative RT-PCR (qRT-PCR) analysis revealed that the expression of AreA was highly induced under nitrogen-limiting conditions. Moreover, compared to wild-type and complemented strains, nitrogen metabolism-related genes were misregulated in ΔareA mutant strains. Pathogenicity assays indicated that the virulence of ΔareA mutant strains were affected by the nitrogen content, but not the carbon content, of fruit hosts. Taken together, our results indicate that CgareA plays a critical role in fungal development, conidia production, regulation of nitrogen metabolism and virulence in Colletotrichum gloeosporioides.
GATA转录因子AreA是一种全局氮调节因子，在微生物中存在优质氮源的情况下，它会限制对复杂和劣质氮源的利用。在本研究中，我们报道了AreA同源物（CgareA基因）在采后果实病原菌胶孢炭疽菌中的生物学功能。areA的靶向基因缺失突变体在营养生长方面显著降低，分生孢子产量增加，分生孢子萌发率略有下降。定量逆转录聚合酶链反应（qRT-PCR）分析表明，AreA的表达在氮限制条件下被高度诱导。此外，与野生型和互补菌株相比，ΔareA突变菌株中氮代谢相关基因的表达失调。致病性测定表明，ΔareA突变菌株的毒力受果实宿主氮含量而非碳含量的影响。综上所述，我们的结果表明CgareA在胶孢炭疽菌的真菌发育、分生孢子产生、氮代谢调节和毒力方面起着关键作用。
