Zhou Zongshan, Wu Jianyuan, Wang Meiyu, Zhang Junxiang
Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, Liaoning, China.
Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, Liaoning, China.
Microb Pathog. 2017 Sep;110:85-92. doi: 10.1016/j.micpath.2017.06.028. Epub 2017 Jun 20.
ATP-binding cassette (ABC) proteins are exclusively found in both prokaryotes and eukaryotes. In this study, we have characterized a gene from Glomerella leaf spot pathogen Colletotrichum gloeosporioides that encodes an ABC protein, whose function to date remains unknown. We designated this gene as CgABCF2. Deletion of CgABCF2 showed drastic reduction both growing rate and conidial production in C. gloeosporioides. The Δcgabcf2 mutant did not form the appressoria, lost the capability to infect apple and failed to form lesions on the wounded leaves and fruits. The C. gloeosporioides native CgABCF2 fully recovered defect of the Δcgabcf2 mutant. These data indicated that CgABCF2 was required for fungal development and invasion. The transcriptions of six pectolytic enzymes genes (CgPG1, CgPG2, pnl-1, pnl-2, pelA and pelB) significantly reduced in the Δcgabcf2 mutant, indicating that deletion of CgABCF2 impaired the fungal necrotrophic growth. In addition, CgABCF2 mediated sexual development through the positive regulation of the gene MAT1-2-1 expression. These results indicated that CgABCF2 underlies the complex process governing morphogenesis, sexual and asexual reproduction, appressorial formation and pathogenicity in C. gloeosporioides.
ATP结合盒（ABC）蛋白仅存在于原核生物和真核生物中。在本研究中，我们鉴定了来自炭疽叶枯病菌胶孢炭疽菌的一个编码ABC蛋白的基因，其功能至今仍不清楚。我们将该基因命名为CgABCF2。CgABCF2的缺失导致胶孢炭疽菌的生长速率和分生孢子产量大幅降低。Δcgabcf2突变体不形成附着胞，失去感染苹果的能力，并且在受伤的叶片和果实上不能形成病斑。胶孢炭疽菌的天然CgABCF2完全恢复了Δcgabcf2突变体的缺陷。这些数据表明CgABCF2是真菌发育和侵染所必需的。在Δcgabcf2突变体中，六个果胶分解酶基因（CgPG1、CgPG2、pnl-1、pnl-2、pelA和pelB）的转录显著降低，表明CgABCF2的缺失损害了真菌的死体营养生长。此外，CgABCF2通过正向调控基因MAT1-2-1的表达介导有性发育。这些结果表明，CgABCF2是胶孢炭疽菌形态发生、有性和无性繁殖、附着胞形成及致病性调控复杂过程的基础。
