Meng Zihao, Zhang Bo, Liu Baosuo, Li Haimei, Fan Sigang, Yu Dahui
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
Fish Shellfish Immunol. 2017 Feb;61:211-218. doi: 10.1016/j.fsi.2016.12.032. Epub 2016 Dec 24.
Carotenoids are a class of natural antioxidants widely found in aquatic, and they have significant effects on the growth, survival, and immunity of these organisms. To investigate the mechanisms of carotenoids in high temperature resistance, we observed the immune response of selected pearl oyster Pinctada fucata (Akoya pearl oyster) families with different carotenoids contents to high temperature stress. The results indicated that the survival rate (SR) of P. fucata decreased significantly with increase in temperature from 26 °C to 34 °C and with the decrease of total carotenoids content (TCC); when the TCC was higher, the SR tended to be higher. TCC and total antioxidant capacity (TAC) decreased significantly at 30 °C with increasing stress time. Correlation analysis indicated that TAC was positively and linearly correlated with TCC, and SR was S-type correlated with TCC and TAC. Immune analysis indicated that levels of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in selected families (with higher TCC) under temperature stress (at 30 °C) were generally significantly lower than in the control group (with lowest TCC) and from 0 to 96 h, the levels of each of these substances varied significantly. Levels of SOD, CAT, and MDA within each family first rose from 0 to 3 h, then decreased to their lowest point after 24 h, and then rose again to their highest levels at 96 h. When TCC was higher, the levels of SOD, CAT, and MDA tended to be lower. These findings indicated that carotenoids play an important role in improving survival rates of P. fucata under high temperature stress by enhancing animals' antioxidant system, and could serve as an index for breeding stress-resistant lines in selective breeding practices.
类胡萝卜素是一类广泛存在于水生生物中的天然抗氧化剂，它们对这些生物的生长、存活和免疫具有重要影响。为了研究类胡萝卜素在耐高温方面的机制，我们观察了具有不同类胡萝卜素含量的选定马氏珠母贝（合浦珠母贝）家系对高温胁迫的免疫反应。结果表明，随着温度从26℃升高到34℃以及总类胡萝卜素含量（TCC）的降低，马氏珠母贝的存活率（SR）显著下降；当TCC较高时，SR往往也较高。在30℃时，随着胁迫时间的增加，TCC和总抗氧化能力（TAC）显著下降。相关性分析表明TAC与TCC呈正线性相关，SR与TCC和TAC呈S型相关。免疫分析表明，在温度胁迫（30℃）下，选定家系（TCC较高）中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）的水平通常显著低于对照组（TCC最低），并且在0至96小时内，这些物质的水平变化显著。每个家系中SOD、CAT和MDA的水平在0至3小时首先上升，然后在24小时后降至最低点，然后在96小时再次升至最高水平。当TCC较高时，SOD、CAT和MDA的水平往往较低。这些发现表明类胡萝卜素通过增强动物的抗氧化系统在提高马氏珠母贝在高温胁迫下的存活率方面发挥重要作用，并且可以作为选择性育种中培育抗逆品系的一个指标。
