Wei Jinfen, Liu Baosuo, Fan Sigang, Zhang Bo, Su Jiaqi, Yu Dahui
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC), South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
Fish Shellfish Immunol. 2017 Mar;62:303-310. doi: 10.1016/j.fsi.2017.01.039. Epub 2017 Jan 31.
The mantle piece from the donor pearl oyster would be rejected by the immune system of recipient oyster in pearl culture practice, especially in the case that the donor and receptor are different species. Thus, investigation of the immune response of recipient oyster to grafted mantle pieces, particularly to xenografts, is of importance in creating xenograft transplantation technology for pearl culture industry. The humoral immune responses of P. fucata to allograft (mantle piece of P. fucata) and xenografts (mantle pieces of P. maxima and P. margaritifera, respectively) were studied in this paper. The oysters receiving no transplantations were served as the control group. The serum was collected from recipient P. fucata at 1 d, 2 d, 3 d, 4 d, 5 d, 7 d, 9 d, 11 d, 13 d, and 15 d, respectively after transplantation, and the serum antibacterial activity, lysozyme activity (LZM), alkaline phosphatase (AKP), acid phosphatase (ACP), total antioxidant capacity (TAC), and agglutination to rabbit red blood cells were investigated. The result indicated that serum of both the experimental groups and the control group can agglutinate rabbit red blood cells, with variation between groups and between time points, respectively. The antibacterial activity in the experimental group was significantly higher than that in the control group at 2-4 d, but lower at 5-11 d and returned back to normal at 15 d, with significant differences among experimental groups (P < 0.05). The LZM in the experimental group was significantly higher than that in the control group at 3-7 d, with significant differences in bacteriolytic activity among various groups (P < 0.05). Both the ACP and AKP activity levels in the experimental groups were higher than those in the control group at 2-9 d, with significant differences among various groups at 3-9 d (P < 0.05). The TAC level in the experimental groups was higher than that in the control group at 1-7 d, with significant differences among various groups at 4-7 d (P < 0.05). The above results indicated that all of the humoral immune factors investigated showed immune responses to both allografts and xenografts, with no specific to any of them. Thus, it is necessary to further screen immune rejection factors specific to xenografts, including cellular immune components.
在珍珠养殖实践中，供体珍珠贝的外套膜组织会被受体牡蛎的免疫系统排斥，尤其是当供体和受体为不同物种时。因此，研究受体牡蛎对移植外套膜组织，特别是异种移植组织的免疫反应，对于为珍珠养殖业创建异种移植技术具有重要意义。本文研究了合浦珠母贝对同种异体移植（合浦珠母贝的外套膜组织）和异种移植（分别为大珠母贝和珠母贝的外套膜组织）的体液免疫反应。未接受移植的牡蛎作为对照组。在移植后第1天、2天、3天、4天、5天、7天、9天、11天、13天和15天，分别从受体合浦珠母贝采集血清，检测血清抗菌活性、溶菌酶活性（LZM）、碱性磷酸酶（AKP）、酸性磷酸酶（ACP）、总抗氧化能力（TAC）以及对兔红细胞的凝集作用。结果表明，实验组和对照组的血清均能凝集兔红细胞，组间和时间点间均存在差异。实验组的抗菌活性在第2 - 4天显著高于对照组，但在第5 - 11天低于对照组，在第15天恢复正常，实验组间差异显著（P < 0.05）。实验组的LZM在第3 - 7天显著高于对照组，各实验组间溶菌活性差异显著（P < 0.05）。实验组的ACP和AKP活性水平在第2 - 9天均高于对照组，在第3 - 9天各实验组间差异显著（P < 0.05）。实验组的TAC水平在第1 - 7天高于对照组，在第4 - 7天各实验组间差异显著（P < 0.05）。上述结果表明，所研究的所有体液免疫因子均对同种异体移植和异种移植表现出免疫反应，且无特异性。因此，有必要进一步筛选异种移植特异性免疫排斥因子，包括细胞免疫成分。
