Li Kun, Lan Yanfang, Luo Houqiang, Shahzad Muhammad, Zhang Hui, Wang Lei, Zhang Lihong, Liu Dongyu, Liu Xinyu, Hao Yanan, Sizhu Suolang, Li Jiakui
Acta Parasitol. 2017 Mar 1;62(1):90-96. doi: 10.1515/ap-2017-0010.
Parasitic nematodes of Oesophagostomum spp., commonly known as 'nodular worms' are one of the most widely distributed and prevalent emerging zoonotic nematodes. However, little is known about the prevalence and gene characteristics of those parasites in Tibetan pigs. Therefore, a study was carried out to investigate the prevalence, isolation and identification of Oesophagostomum spp from Tibetan pigs by genetic markers of nad1,cox3 and ITS1 for the first time. The results revealed that the infection rate of O. dentatum and O. quadrispinulatum by genetic markers of nad1 was 81.13%; 35 (66.04%); the O. dentatum and O. quadrispinulatum by genetic markers cox3 was 66.04%, and O. dentatum and O. stephanostomum by genetic markers ITS1 was found to be 77.36%. Interestingly, the O. stephanostomum specie was identified and isolated from 90.48% stomach and 69.23% colon samples by genetic markers of ITS1. The present study, for the first time has described the presence and genetic characterization of Oesophagostomun spp of O. dentatum, O. quadrispinulatum and especially O. stephanostomum in Tibetan pigs from the high and remote Tibetan plateau. A public concern should be raised in terms of economical losses and severe public health problem.
食道口线虫属的寄生线虫，通常被称为“结节虫”，是分布最广、最普遍的新兴人畜共患线虫之一。然而，对于这些寄生虫在藏猪中的流行情况和基因特征知之甚少。因此，首次通过nad1、cox3和ITS1的基因标记对藏猪中的食道口线虫属进行了流行情况调查、分离和鉴定。结果显示，通过nad1基因标记，齿状食道口线虫和四棘食道口线虫的感染率分别为81.13%和35（66.04%）；通过cox3基因标记，齿状食道口线虫和四棘食道口线虫的感染率为66.04%，通过ITS1基因标记，齿状食道口线虫和斯蒂芬氏食道口线虫的感染率为77.36%。有趣的是，通过ITS1基因标记，在90.48%的胃样本和69.23%的结肠样本中鉴定并分离出了斯蒂芬氏食道口线虫。本研究首次描述了来自高海拔偏远青藏高原藏猪中齿状食道口线虫、四棘食道口线虫，尤其是斯蒂芬氏食道口线虫的存在及其基因特征。在经济损失和严重的公共卫生问题方面应引起公众关注。
