Yang Qian, Zhang Yong, Yan Dongmei, Zhu Shuangli, Wang Dongyan, Ji Tianjiao, Li Xiaolei, Song Yang, Gu Xinrui, Xu Wenbo
WHO WPRO Regional Polio Reference Laboratory and Key Laboratory of Medical Virology, National Health and Family Planning Commission of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China.
PLoS One. 2016 Dec 28;11(12):e0169021. doi: 10.1371/journal.pone.0169021. eCollection 2016.
Coxsackievirus A2 (CV-A2) has been frequently detected and commonly associated with hand, foot, and mouth disease (HFMD) in China since 2008. However, limited sequences of CV-A2 are currently available. As a result, we have been focusing on the genetic characteristics of CV-A2 in the mainland of China during 2008-2015 based on national HFMD surveillance. In this study, 20 CV-A2 strains were isolated and phylogenetic analyses of the VP1 sequences were performed. Full-length genome sequences of two representative CV-A2 isolates were acquired and similarity plot and bootscanning analyses were performed. The phylogenetic dendrogram indicated that all CV-A2 strains could be divided into four genotypes (Genotypes A-D). The CV-A2 prototype strain (Fleetwood) was the sole member of genotype A. From 2008 to 2015, the CV-A2 strains isolated in China dispersed into two different genotypes (B and D). And the genotype D became the dominant circulating strains in China. Strains isolated in Russia and India from 2005 to 2011 converged into genotype C. Intertypic recombination occurred between the Chinese CV-A2 strains and other enterovirus-A donor sequences. This result reconfirmed that recombination is a common phenomenon among enteroviruses. This study helps expand the numbers of whole virus genome sequence and entire VP1 sequence of CV-A2 in the GenBank database for further researcher.
自2008年以来，柯萨奇病毒A2型（CV-A2）在中国频繁被检测到，且通常与手足口病（HFMD）相关。然而，目前CV-A2的序列有限。因此，基于全国手足口病监测，我们一直关注2008 - 2015年中国大陆CV-A2的基因特征。在本研究中，分离出20株CV-A2毒株，并对VP1序列进行了系统发育分析。获得了两株代表性CV-A2分离株的全长基因组序列，并进行了相似性图谱和靴带扫描分析。系统发育树状图表明，所有CV-A2毒株可分为四个基因型（A - D型）。CV-A2原型株（弗利特伍德）是A型的唯一成员。2008年至2015年，在中国分离的CV-A2毒株分散为两个不同的基因型（B型和D型）。并且D型成为中国主要的流行毒株。2005年至2011年在俄罗斯和印度分离的毒株汇聚为C型。中国CV-A2毒株与其他肠道病毒A供体序列之间发生了型间重组。这一结果再次证实重组是肠道病毒中的常见现象。本研究有助于扩充GenBank数据库中CV-A2的全病毒基因组序列和完整VP1序列数量，以供进一步研究。
