Wang Xiaoguang, Ouyang Lin, Luo Lingfei, Liu Jiqian, Song Chiping, Li Cuizhen, Yan Hongjing, Wang Ping
The Center for Disease Control and Prevention of Minghang District, Minghang District, Shanghai 201101, P.R. China.
Oncotarget. 2017 Jan 24;8(4):6079-6084. doi: 10.18632/oncotarget.14036.
Methicillin-resistant Staphylococcus aureus (MRSA) strains are now common both in the health care setting and in the community. Active surveillance is critical for MRSA control and prevention. Specimens of patients (200 patients with 1119 specimens) as well as medical staff and hospital setting (1000 specimens) were randomly sampled in a level 2 hospital in Shanghai from September 2011 to August 2012. Isolation, cultivation and identification of S. aureus were performed. Totally, 67 S. aureus strains were isolated. 32 S. aureus strains were isolated from patient samples; 13 (13/32, 40.6%) of the 32 S. aureus isolates were MRSA; sputum sample and patients in the department of general internal medicine were the most frequent specimen and patient group for S. aureus strains isolation. Remaining 35 S. aureus strains were isolated from the medical staff and hospital setting; 20 (20/35, 57.1%) of the 35 S. aureus isolates were MRSA; specimens sampled from doctors and nurses' hands and nose and hospital facilities were the most frequent samples to isolate S. aureus. Resistant and virulent genes detection showed that, all 33 MRSA strains were mecA positive which accounts for 49.3% of the 67 S. aureus strains; 38 isolates were Panton-Valentine leukocidin (PVL) gene positive which accounts for 56.7% of the 67 S. aureus strains; and 17 (17/67, 25.4%) isolates are mecA and PVL genes dual positive. Multidrug-resistant strains of MRSA and PVL positive S. aureus are common in patients, medical staff and hospital setting, the potential health threat is worthy of our attention.
耐甲氧西林金黄色葡萄球菌（MRSA）菌株如今在医疗环境和社区中都很常见。主动监测对于MRSA的控制和预防至关重要。2011年9月至2012年8月期间，在上海一家二级医院对患者标本（200例患者，共1119份标本）以及医务人员和医院环境（1000份标本）进行了随机抽样。进行了金黄色葡萄球菌的分离、培养和鉴定。共分离出67株金黄色葡萄球菌。从患者样本中分离出32株金黄色葡萄球菌；32株金黄色葡萄球菌分离株中有13株（13/32，40.6%）为MRSA；痰液样本和普通内科患者是分离金黄色葡萄球菌菌株最常见的标本和患者群体。其余35株金黄色葡萄球菌从医务人员和医院环境中分离得到；35株金黄色葡萄球菌分离株中有20株（20/35，57.1%）为MRSA；从医生和护士的手、鼻以及医院设施中采集的标本是分离金黄色葡萄球菌最常见的样本。耐药和毒力基因检测显示，所有33株MRSA菌株mecA均为阳性，占67株金黄色葡萄球菌菌株的49.3%；38株分离株Panton-Valentine杀白细胞素（PVL）基因阳性，占67株金黄色葡萄球菌菌株的56.7%；17株（17/67，25.4%）分离株mecA和PVL基因双阳性。MRSA多重耐药菌株和PVL阳性金黄色葡萄球菌在患者、医务人员和医院环境中很常见，其潜在的健康威胁值得我们关注。
