Fateh Shirin, Dibazar Shaghayegh Pishkhan, Daneshmandi Saeed
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Future Sci OA. 2015 Nov 1;1(4):FSO49. doi: 10.4155/fso.15.49. eCollection 2015 Nov.
Food and medicinal applications of barberry date back to 2500 years ago. This study investigates impact on lymphocytic immune responses.
MATERIALS & METHODS: Balb/c splenocytes were treated by 0.001-1000 μg/ml of aqueous and alcoholic extracts in presence of phytohemagglutinin and lipopolysaccharide mitogens. Cell proliferation was assayed and cytokines were measured using ELISA.
Both extracts suppressed proliferation of phytohemagglutinin stimulated splenocytes (as T cells), while alcoholic extract induced expansion of lipopolysaccharide activated cells (as B lymphocytes) and unstimulated cells (p < 0.05). Both barberry extracts suppressed IFN-γ production (p < 0.05) and enhanced IL-4, IL-10 and TGF-β release from splenocytes (p < 0.05).
Both extracts could suppress T-cell and enhance B-cell proliferation and shift immune responses toward Th2.
伏牛花的食品和药用应用可追溯到2500年前。本研究调查其对淋巴细胞免疫反应的影响。
在植物血凝素和脂多糖促细胞分裂剂存在的情况下，用0.001 - 1000μg/ml的水提取物和醇提取物处理Balb/c脾细胞。测定细胞增殖情况，并使用酶联免疫吸附测定法测量细胞因子。
两种提取物均抑制植物血凝素刺激的脾细胞（作为T细胞）的增殖，而醇提取物诱导脂多糖激活的细胞（作为B淋巴细胞）和未刺激细胞的扩增（p < 0.05）。两种伏牛花提取物均抑制γ-干扰素的产生（p < 0.05），并增强脾细胞中白细胞介素-4、白细胞介素-10和转化生长因子-β的释放（p < 0.05）。
两种提取物均可抑制T细胞并增强B细胞增殖，并使免疫反应向Th2型转变。
