Sun Jun-Ling, Hu Yuan-Liang, Wang De-Yun, Zhang Bao-Kang, Liu Jia-Guo
Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
Vaccine. 2006 Mar 20;24(13):2343-8. doi: 10.1016/j.vaccine.2005.11.053. Epub 2005 Dec 9.
Two compound Chinese herbal medicinal ingredients (cCIs) were prepared respectively with epimedium polysaccharide (EPS) plus propolis flavone (PF) and astragalus polysaccharide (APS) plus ginsenoside (GS). Also, two compound Chinese herbal medicines (cCMs) with the same ingredient content as corresponding cCIs were made with the extracts of epimedium plus propolis and astragalus plus ginseng. In rabbit immune trial, two cCIs, physiological saline in the control, were respectively injected to the rabbits vaccinated with inactivated rabbit hemorrhagic disease vaccine. On Days 3, 7, 10, 14, 21, 28, 35 and 42 after vaccination, the dynamic changes of serum antibody titers were determined by hemagglutination inhibition (HI) test. In chicken immune trial, all of cCIs and cCMs were mixed respectively with inactivated Newcastle disease vaccine virus to vaccinate chickens, taking oil-adjuvant and non-adjuvant vaccine as controls. On Days 7, 14, 21, 28, 35 and 42 after vaccination, the dynamic changes of peripheral lymphocyte proliferation and serum antibody titers were tested respectively by MTT method and HI test method. The results showed that both cCIs could significantly raise antibody titer in rabbits, which the effect of compound Chinese herbal medicinal ingredients 1 (cCIs 1) was better than that of compound Chinese herbal medicinal ingredients 2 (cCIs 2). All of cCIs and cCMs could markedly promote lymphocyte proliferation and enhance antibody titer in chickens, which was similar to oil adjuvant, the immunologic enhancement of cCIs were slightly superior to that of the cCMs.
分别用淫羊藿多糖（EPS）加蜂胶黄酮（PF）以及黄芪多糖（APS）加人参皂苷（GS）制备了两种复合中草药成分（cCIs）。还用淫羊藿加蜂胶提取物以及黄芪加人参提取物制成了两种与相应cCIs成分含量相同的复合中草药（cCMs）。在兔免疫试验中，将两种cCIs以及作为对照的生理盐水分别注射到接种了兔出血症灭活疫苗的兔子体内。在接种疫苗后的第3、7、10、14、21、28、35和42天，通过血凝抑制（HI）试验测定血清抗体效价的动态变化。在鸡免疫试验中，将所有cCIs和cCMs分别与新城疫灭活疫苗病毒混合后给鸡接种，以油佐剂疫苗和非佐剂疫苗作为对照。在接种疫苗后的第7、14、21、28、35和42天，分别通过MTT法和HI试验法检测外周淋巴细胞增殖和血清抗体效价 的动态变化。结果表明，两种cCIs均可显著提高兔子的抗体效价，其中复合中草药成分1（cCIs 1）的效果优于复合中草药成分2（cCIs 2）。所有cCIs和cCMs均可显著促进鸡的淋巴细胞增殖并提高抗体效价，这与油佐剂相似，cCIs的免疫增强作用略优于cCMs。
