Mursalimov Sergey, Sidorchuk Yuriy, Deineko Elena
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
Cell Biol Int. 2017 Mar;41(3):340-344. doi: 10.1002/cbin.10718. Epub 2017 Jan 6.
Behavior of nucleolus during the nuclear migration between plant cells (cytomixis) is studied for the first time in the tobacco male meiosis. As is shown, the nucleolus is located in a nonrandom manner in the migrating nuclei. In the majority of cases, the nucleolus resides on the nuclear pole strictly opposite to the cytomictic channel. Owing to this localization, the nucleolus extremely rare enters the recipient cell, so that the nucleolar material is in most cases undetectable in the micronuclei formed after cytomixis. When a whole nucleus migrates from a donor cell to recipient, the nucleolus can leave the nucleus and remain in the donor cells either alone or with a small amount of chromatin. The causes underlying a nonrandom location of the nucleolus in cytomictic cells are discussed. It is assumed that the nucleolar material contacts the cytoplasmic cytoskeleton, which prevents migration of the nucleolus into another cell within the nucleus. The potential use of cytomixis as a model for studying the nuclear motion is discussed.
首次在烟草雄性减数分裂过程中研究了植物细胞间核迁移（细胞融合）过程中核仁的行为。结果表明，核仁在迁移的细胞核中以非随机方式定位。在大多数情况下，核仁位于与细胞融合通道严格相对的核极上。由于这种定位，核仁极少进入受体细胞，因此在细胞融合后形成的微核中，大多数情况下检测不到核仁物质。当整个细胞核从供体细胞迁移到受体细胞时，核仁可以离开细胞核，单独或携带少量染色质留在供体细胞中。讨论了细胞融合细胞中核仁非随机定位的潜在原因。据推测，核仁物质与细胞质细胞骨架接触，这阻止了核仁迁移到细胞核内的另一个细胞中。还讨论了将细胞融合作为研究核运动模型的潜在用途。
