Mursalimov Sergey, Sidorchuk Yuri, Deineko Elena
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 10, Novosibirsk, 630090, Russian Federation.
Protoplasma. 2017 Jan;254(1):539-545. doi: 10.1007/s00709-016-0973-z. Epub 2016 Apr 12.
Confocal laser scanning microscopy for the first time is used to examine the structure of the tobacco microsporocytes involved in the intercellular migration of nuclei (cytomixis). As is observed, the cytomictic channels are distributed over the surface of tobacco microsporocytes in a non-random manner and their number depends on the meiotic stage. Analysis of non-squash cells demonstrates the differences in cytological patterns of cytomixis in a normal meiosis of control tobacco plants (SR1 line) and the abnormal meiosis of polyploids. As a rule, two to three adjacent cells are involved in cytomixis during meiosis of control tobacco plants; after cytomixis, several micronuclei are formed in recipient cells; cytoplasts (enucleated cells) are rare; and polyads are undetectable. In the meiosis of polyploids, cytomixis is massive, with a larger number of cells (sometimes, over ten) involved in nuclear migration simultaneously; recipient cells on completion of cytomixis develop tens of micronuclei; cytoplasts and polyads are frequently detectable.
共聚焦激光扫描显微镜首次用于研究参与细胞核细胞间迁移（细胞融合）的烟草小孢子母细胞的结构。可以观察到，细胞融合通道以非随机方式分布在烟草小孢子母细胞表面，其数量取决于减数分裂阶段。对非压片细胞的分析表明，对照烟草植株（SR1品系）正常减数分裂和多倍体异常减数分裂中细胞融合的细胞学模式存在差异。通常，在对照烟草植株减数分裂期间，两到三个相邻细胞参与细胞融合；细胞融合后，受体细胞中会形成几个微核；细胞质体（无核细胞）很少见；四分体不可检测。在多倍体的减数分裂中，细胞融合大量发生，同时有大量细胞（有时超过十个）参与核迁移；细胞融合完成后，受体细胞会形成数十个微核；细胞质体和四分体经常可以检测到。
