Shimomura O, Musicki B, Kishi Y
Marine Biological Laboratory, Woods Hole, MA 02543.
Biochem J. 1989 Aug 1;261(3):913-20. doi: 10.1042/bj2610913.
Thirty-seven coelenterazine analogues were synthesized and incorporated into apo-aequorin, yielding 30 semi-synthetic aequorins that have the capacity to emit a significant amount of light in the presence of Ca2+. The properties of resultant photoproteins were investigated. The most prominent feature of those photoproteins was the wide range in their sensitivities to Ca2+ concentration. The relative intensity of Ca2+-triggered luminescence of the photoproteins ranged from 0.01 to 190 when compared with natural aequorin (relative intensity 1.0) at pCa 6 for the cases where the relative intensity is less than 1 and at pCa 7 for the cases where the relative intensity is higher than 1. Eight of the semi-synthetic aequorins belonged to the class of e-aequorin. With two of those photoproteins, the degree of dependence of the luminescence intensity ratio I400/I465 on pCa was greater than that with e-aequorin, suggesting that these two photoproteins are possibly superior to e-aequorin in measuring Ca2+ concentration by the ratio method.
合成了37种腔肠素类似物，并将其掺入脱辅基水母发光蛋白中，得到了30种半合成水母发光蛋白，它们在Ca2+存在时能够发出大量的光。对所得光蛋白的性质进行了研究。这些光蛋白最显著的特点是它们对Ca2+浓度的敏感性范围很广。与天然水母发光蛋白（相对强度为1.0）相比，在pCa 6时，相对强度小于1的情况下，光蛋白由Ca2+触发的发光相对强度范围为0.01至190；在pCa 7时，相对强度大于1的情况下，光蛋白由Ca2+触发的发光相对强度范围为0.01至190。八种半合成水母发光蛋白属于e型水母发光蛋白。其中两种光蛋白的发光强度比I400/I465对pCa的依赖程度大于e型水母发光蛋白，这表明这两种光蛋白在用比率法测量Ca2+浓度方面可能优于e型水母发光蛋白。
