哈利斯塔林、菲阿利定以及作为生物系统中钙离子指示剂的水母发光蛋白的修饰形式。
Halistaurin, phialidin and modified forms of aequorin as Ca2+ indicator in biological systems.
作者信息
Shimomura O, Shimomura A
出版信息
Biochem J. 1985 Jun 15;228(3):745-9. doi: 10.1042/bj2280745.
Abstract
Two kinds of aequorin-type photoproteins, i.e., halistaurin and phialidin, and four kinds of modified forms of aequorin, i.e., products of acetylation, ethoxycarbonylation, fluorescamine-modification and fluorescein labelling, were prepared. The modified forms of aequorin were more sensitive to Ca2+ than was aequorin in their Ca2+-triggered luminescence reactions, whereas halistaurin and phialidin were less sensitive. The emission maxima of luminescence were all within a wavelength range 450-464 nm, except for fluorescein-labelled aequorin, which emitted yellowish light (lambda max. 520 nm). A new technique of measuring Ca2+ concentration is suggested.
摘要
制备了两种水母发光蛋白类型，即海葵荧光蛋白和海鳃荧光蛋白，以及四种水母发光蛋白的修饰形式，即乙酰化、乙氧羰基化、荧光胺修饰和荧光素标记的产物。在其Ca2+触发的发光反应中，水母发光蛋白的修饰形式对Ca2+比水母发光蛋白更敏感，而海葵荧光蛋白和海鳃荧光蛋白则较不敏感。除了发出淡黄色光（最大波长520nm）的荧光素标记的水母发光蛋白外，发光的最大发射波长均在450 - 464nm范围内。提出了一种测量Ca2+浓度的新技术。
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2
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3
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