5,6-二氯-1-β-D-呋喃核糖基苯并咪唑对小牛胸腺酪蛋白激酶II的抑制动力学
Kinetics of inhibition by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole on calf thymus casein kinase II.
作者信息
Zandomeni R O
机构信息
Comparative Leukemia Studies Unit, New Bolton Center, University of Pennsylvania, Kennett Square 19348.
出版信息
Biochem J. 1989 Sep 1;262(2):469-73. doi: 10.1042/bj2620469.
Abstract
The adenosine analogue 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) is a specific inhibitor for RNA polymerase II transcription in vivo and in vitro [Tamm + Sehgal (1978) Adv. Virus Res. 22, 187-258; Zandomeni & Weinmann (1984) J. Biol. Chem. 259, 14804-14811]. The effect on RNA polymerase II-specific transcription seems to be mediated by its inhibition of nuclear casein kinase II [Zandomeni, Carrera-Zandomeni, Shugar & Weinmann (1986) J. Biol. Chem. 261, 3414-3419]. Inhibition studies indicated that DRB acted as a mixed-type inhibitor with respect to casein and as a competitive inhibitor with respect to the nucleotide phosphate donor substrates. The DRB inhibition constant is 7 microM for the calf thymus casein kinase II, with regard to both ATP and GTP.
摘要
腺苷类似物5,6-二氯-1-β-D-呋喃核糖基苯并咪唑（DRB）是体内和体外RNA聚合酶II转录的特异性抑制剂[Tamm + Sehgal（1978年）《病毒研究进展》22，187 - 258；Zandomeni & Weinmann（1984年）《生物化学杂志》259，14804 - 14811]。对RNA聚合酶II特异性转录的影响似乎是通过其对核酪蛋白激酶II的抑制作用介导的[Zandomeni，Carrera - Zandomeni，Shugar & Weinmann（1986年）《生物化学杂志》261，3414 - 3419]。抑制研究表明，DRB对酪蛋白而言是混合型抑制剂，对核苷酸磷酸供体底物而言是竞争性抑制剂。就ATP和GTP而言，小牛胸腺酪蛋白激酶II的DRB抑制常数为7微摩尔。
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本文引用的文献
1
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J Biol Chem. 1980 May 25;255(10):4589-94.
2
A heparin-sensitive nuclear protein kinase. Purification, properties, and increased activity in rat hepatoma relative to liver.一种对肝素敏感的核蛋白激酶。纯化、性质以及与肝脏相比大鼠肝癌中活性的增加。
J Biol Chem. 1981 Jul 25;256(14):7468-77.
3
Mechanism of action of dichloro-beta-D-ribofuranosylbenzimidazole: effect on in vitro transcription.二氯-β-D-呋喃核糖基苯并咪唑的作用机制：对体外转录的影响
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4
Cyclic nucleotide independent casein kinase (G type) in bovine adrenal cortex: purification and properties of two molecular forms.牛肾上腺皮质中不依赖环核苷酸的酪蛋白激酶（G型）：两种分子形式的纯化及特性
Biochim Biophys Acta. 1981 Apr 14;658(2):191-201. doi: 10.1016/0005-2744(81)90289-8.
5
Purification and properties of calf thymus casein kinases I and II.小牛胸腺酪蛋白激酶I和II的纯化及性质
J Biol Chem. 1981 Apr 10;256(7):3319-25.
6
7
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