Pohle W, Räim T
Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of the GDR, Jena.
Biomed Biochim Acta. 1989;48(7):377-86.
Infrared linear dichroism and X-ray diffraction analysis on oriented films and fibres, respectively, were used simultaneously to elucidate structural characteristics of a DNA-drug complex (the drug was the anticancer antibiotic violamycin BI, an anthracycline derivative). First, the parameters accessible by the two methods are briefly explained; then, attempts to inter-correlate the data from both lines of investigations are made, which demonstrate that they support and supplement each other favourably concerning several structural aspects. This holds, in particular, for results regarding the conformational flexibility of DNA in complexes and the relative amounts of intercalated and outside-bound drug molecules. Thus, the study represents an example for a successful combined application of two physical methods to one biochemical problem.
分别对取向薄膜和纤维进行红外线性二色性和X射线衍射分析，同时用于阐明DNA-药物复合物（药物为抗癌抗生素紫霉素BI，一种蒽环类衍生物）的结构特征。首先，简要解释两种方法可获取的参数；然后，尝试将两条研究路线的数据相互关联，结果表明它们在几个结构方面相互支持且相互补充。这尤其适用于关于复合物中DNA构象灵活性以及嵌入和外部结合药物分子相对数量的结果。因此，该研究是将两种物理方法成功联合应用于一个生化问题的一个实例。
