从牛阴茎退缩肌（BRP）产生活性平滑肌抑制因子（IF）的机制。

Mechanism for the generation of active smooth muscle inhibitory factor (IF) from bovine retractor penis muscle (BRP).

作者信息

Yui Y, Ohkawa S, Ohnishi K, Hattori R, Aoyama T, Takahashi M, Morishita H, Terao Y, Kawai C

机构信息

Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.

出版信息

Biochem Biophys Res Commun. 1989 Oct 16;164(1):544-9. doi: 10.1016/0006-291x(89)91753-1.

DOI:10.1016/0006-291x(89)91753-1

PMID:2803318

Abstract

The active smooth muscle inhibitory factor (IF) is generated by acidifying the extracts from the bovine retractor penis muscle (BRP). The activated IF is very similar to endothelium-derived relaxing factor (EDRF). However, the mechanism for the generation of active IF is yet unknown. From reverse-phase high performance liquid chromatographic data, the activities of nitrite loss were closely related to the contents of both sulfhydryl (SH) groups and L-cysteine. Thus, under acidic conditions, RSH and nitrite in the extracts are considered to react as follows: RSH + HNO2----RSNO + H2O, RSNO----(1/2) RSSR + NO, NO + (1/2)O2----NO2, NO2 + (1/2)H2O----(1/2)HNO2 + (1/2)HNO3. In addition to NO, RSNO is also a labile but potent vasodilator. Thus, activated IF seems to consist of RSNO and NO, and thiol-containing small molecules (molecular weight of less than 1,000) from BRP are closely involved in the generation of active IF.

摘要

活性平滑肌抑制因子（IF）是通过酸化牛阴茎退缩肌（BRP）提取物产生的。活化的IF与内皮源性舒张因子（EDRF）非常相似。然而，活性IF的产生机制尚不清楚。从反相高效液相色谱数据来看，亚硝酸盐损失的活性与巯基（SH）基团和L-半胱氨酸的含量密切相关。因此，在酸性条件下，提取物中的RSH和亚硝酸盐被认为按以下方式反应：RSH + HNO2→RSNO + H2O，RSNO→(1/2) RSSR + NO，NO + (1/2)O2→NO2，NO2 + (1/2)H2O→(1/2)HNO2 + (1/2)HNO3。除了NO，RSNO也是一种不稳定但有效的血管舒张剂。因此，活化的IF似乎由RSNO和NO组成，并且来自BRP的含硫醇小分子（分子量小于1000）密切参与活性IF的产生。