Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, China.
Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, Kentucky, 40292.
Dev Neurobiol. 2017 Sep;77(8):907-916. doi: 10.1002/dneu.22476. Epub 2017 Mar 23.
Mouse primary oligodendrocyte precursor cells (OPCs) are increasingly used to study the molecular mechanisms underlying the phenotype changes in oligodendrocyte differentiation and axonal myelination observed in transgenic or mutant mouse models. However, mouse OPCs are much more difficult to be isolated by the simple dissociation culture of brain tissues than their rat counterparts. To date, the mechanisms underlying the species difference in OPC preparation remain obscure. In this study, we showed that astrocytes from rats have a stronger effect than those from mouse in promoting OPC proliferation and survival in vitro. Mouse astrocytes displayed significantly weaker viability in culture and reduced potential in maintaining OPC self-renewal, as confirmed by culturing OPCs with conditioned media from rat or mouse astrocytes. These results explained the reason for why stratified cultures of OPCs and astrocytes are difficult to be achieved in mouse CNS tissues. Based on these findings, we adopted inactivated rat astrocytes as feeder cells to support the self-renewal of mouse cortical OPCs and preparation of high-purity mouse OPCs. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 77: 907-916, 2017.
鼠源初代少突胶质前体细胞（OPC）被越来越多地用于研究在转基因或突变体鼠模型中观察到的少突胶质分化和轴突髓鞘形成的表型变化的分子机制。然而，与大鼠相比，通过简单的脑组织解离培养来分离鼠源 OPC 要困难得多。迄今为止，OPC 制备中种属差异的机制仍不清楚。在本研究中，我们发现大鼠来源的星形胶质细胞比小鼠来源的星形胶质细胞在体外促进 OPC 增殖和存活方面具有更强的作用。通过用大鼠或小鼠星形胶质细胞的条件培养基培养 OPC，证实了鼠源星形胶质细胞在培养中具有明显较弱的活力和降低的维持 OPC 自我更新的潜能。这些结果解释了为什么在鼠中枢神经系统组织中难以实现 OPC 和星形胶质细胞的分层培养。基于这些发现，我们采用失活的大鼠星形胶质细胞作为饲养细胞，以支持鼠皮质 OPC 的自我更新和高纯度鼠 OPC 的制备。Wiley Periodicals, Inc. 2017 版权所有。发展神经生物学 77:907-916。
