Shahi Heshmat, Bahreiny Rasoul, Reiisi Somayeh
Department of Immunology, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Internal Medicine, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Acta Med Iran. 2016 Nov;54(11):704-708.
Helicobacter Pylori infection is a common gastrointestinal infection that can cause pathological effects, increase oxidative stress and induce an inflammatory response in gastric mucosa. Inflammatory aspects may prompt the production of radical oxygen substance (ROS) which may damage cells and release 8-hydroxydyoxyguanosine (8-OHdG) to serum. In this study, we evaluate the prevalence of H. pylori virulence factors and the association between serum level of 8-OHdG, H. pylori infection, and its various virulence factors. The presence of H. pylori and prevalence of cagA, babA and oipA genes in samples were determined by rapid urease test (RUT), histopathological exam (HE) and polymerase chain reaction (PCR) and oxidative DNA damage situation were assessed by using serum level of 8-OHdG. There was not any direct relation between H. pylori negative and H. pylori oipA+specimens by 8-OHdG serum level (P>0.05). In all clinical observations, the presence of cagA and oipA genes was common. There was a statistical relationship between the presence of cagA, babA factors, and high serum level of 8-OHdG (P<0.05). The presence of cagA and babA virulence factors may be associated with increased serum 8-OHdG in dyspeptic patients and may induce the damage to gastric cells.
幽门螺杆菌感染是一种常见的胃肠道感染，可引起病理效应，增加氧化应激并诱导胃黏膜发生炎症反应。炎症方面可能促使活性氧物质（ROS）产生，ROS可能会损伤细胞并导致8-羟基脱氧鸟苷（8-OHdG）释放到血清中。在本研究中，我们评估了幽门螺杆菌毒力因子的流行情况以及血清8-OHdG水平、幽门螺杆菌感染及其各种毒力因子之间的关联。通过快速尿素酶试验（RUT）、组织病理学检查（HE）和聚合酶链反应（PCR）测定样本中幽门螺杆菌的存在情况以及cagA、babA和oipA基因的流行情况，并使用血清8-OHdG水平评估氧化DNA损伤情况。8-OHdG血清水平在幽门螺杆菌阴性和幽门螺杆菌oipA+样本之间没有任何直接关系（P>0.05）。在所有临床观察中，cagA和oipA基因的存在很常见。cagA、babA因子的存在与血清8-OHdG高水平之间存在统计学关系（P<0.05）。cagA和babA毒力因子的存在可能与消化不良患者血清8-OHdG升高有关，并可能导致胃细胞损伤。
