Shang Ying-Ying, Qin Qin, Li Meng-Shun, Xu Hai-Yan, Ge Yue-Bin
a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
Nat Prod Res. 2017 May;31(9):1066-1072. doi: 10.1080/14786419.2016.1272109. Epub 2016 Dec 29.
The leaves of Elaeagnus pungens were extracted and successively purified by a set chromatographic techniques. The structures of the obtained constituents were elucidated on the basis of spectroscopic methods including 1D/2D NMR and high resolution-MS. One new flavonoid glycosides, kaempferol 3-O-β-d-glucopyranosyl-(1 → 3)-α-l-rhamnopyranosyl-(1 → 6)-[α-l-rhamnopyranosyl(1 → 2)]-β-d-galactopyranoside-7-O-β-d-glucopyranoside (1), along with five known compounds (2-6) were isolated and identified. The MTT assay in vitro showed that the isolated flavonol glycosides have no proliferating activity, suggesting no aggravating impact on the increased airway smooth muscle cells.
对胡颓子叶进行提取，并通过一系列色谱技术依次纯化。基于包括一维/二维核磁共振和高分辨质谱在内的光谱方法阐明了所得成分的结构。分离并鉴定出一种新的黄酮苷，山柰酚3 - O - β - d - 吡喃葡萄糖基 - (1→3) - α - l - 吡喃鼠李糖基 - (1→6) - [α - l - 吡喃鼠李糖基(1→2)] - β - d - 吡喃半乳糖苷 - 7 - O - β - d - 吡喃葡萄糖苷（1），以及五种已知化合物（2 - 6）。体外MTT试验表明，分离得到的黄酮醇苷没有增殖活性，表明对气道平滑肌细胞增多没有加重影响。
