Lu Yanfen, Chen Qi, Bu Yufen, Luo Rui, Hao Suxiao, Zhang Jie, Tian Ji, Yao Yuncong
Plant Science and Technology College, Beijing University of AgricultureBeijing, China.
Beijing Key Laboratory for Agricultural Applications and New TechniquesBeijing, China.
Front Plant Sci. 2017 Jul 18;8:1286. doi: 10.3389/fpls.2017.01286. eCollection 2017.
Cedar-apple rust ( Miyabe) is a fungal disease that causes substantial injury to apple trees and results in fruit with reduced size and quality and a lower commercial value. The molecular mechanisms underlying the primary and secondary metabolic effects of rust spots on the leaves of apple cultivars are poorly understood. Using HPLC, we found that the contents of flavonoid compounds, especially anthocyanin and catechin, were significantly increased in rust-infected symptomatic tissue (RIT). The expression levels of structural genes and MYB transcription factors related to flavonoid biosynthesis were one- to seven-fold higher in the RIT. Among these genes, and showed more than a 10-fold increase, suggesting that these genes were expressed at significantly higher levels in the RIT. Hormone concentration assays showed that the levels of abscisic acid (ABA), ethylene (ETH), jasmonate (JA) and salicylic acid (SA) were higher in the RIT and were consistent with the expression levels of and , respectively. Our study explored the complicated crosstalk of the signal transduction pathways of ABA, ETH, JA and SA; the primary metabolism of glucose, sucrose, fructose and sorbitol; and the secondary metabolism of flavonoids involved in the rust resistance of crabapple leaves.
苹果桧锈病（宫部）是一种真菌病害，会对苹果树造成严重损害，导致果实大小和品质下降，商业价值降低。锈斑对苹果品种叶片的初级和次级代谢影响的分子机制尚不清楚。通过高效液相色谱法，我们发现黄酮类化合物，尤其是花青素和儿茶素的含量在感染锈病的有症状组织（RIT）中显著增加。与黄酮类生物合成相关的结构基因和MYB转录因子的表达水平在RIT中高出1至7倍。在这些基因中， 和 显示出超过10倍的增加，表明这些基因在RIT中的表达水平显著更高。激素浓度测定表明，脱落酸（ABA）、乙烯（ETH）、茉莉酸（JA）和水杨酸（SA）的水平在RIT中较高，分别与 和 的表达水平一致。我们的研究探讨了ABA、ETH、JA和SA信号转导途径的复杂相互作用；葡萄糖、蔗糖、果糖和山梨醇的初级代谢；以及海棠叶锈病抗性中涉及的黄酮类次级代谢。
