Rossi G A, Sacco O, Vassallo F, Lantero S, Morelli A, Benatti U, Damiani G
First Division of Pneumology, San Martino Hospital, Genoa, Italy.
Eur Respir J Suppl. 1989 Jun;6:435s-440s.
Eosinophils like neutrophils possess an oxidative metabolism which is activated by phagocytosis and cell membrane activation. Since eosinophils may take part in inflammatory reactions by releasing oxygen species and have membrane receptors for immunoglobulins, immune complexes may activate their respiratory burst. We compared the ability of phorbol myristate acetate (PMA) and immunoglobulin G (IgG) and immunoglobulin E (IgE) immune complexes to stimulate hydrogen peroxide (H2O2) release from circulating eosinophils and neutrophils. PMA was able to stimulate a significant respiratory burst in both cell types, PMA-induced H2O2 production being higher in eosinophils than in neutrophils (p less than 0.05). Similar results were obtained when the cells were stimulated by IgG (p less than 0.05). In contrast, aggregated human myeloma IgE induced a detectable H2O2 release only from peripheral blood eosinophils. We conclude that H2O2 release from eosinophils may play a role in parenchymal injury associated with hypereosinophilic disorders and the presence of immune complexes at sites of disease activity.
嗜酸性粒细胞与中性粒细胞一样，具有一种通过吞噬作用和细胞膜激活而被激活的氧化代谢。由于嗜酸性粒细胞可能通过释放氧物质参与炎症反应，并且具有免疫球蛋白的膜受体，免疫复合物可能激活其呼吸爆发。我们比较了佛波酯（PMA）、免疫球蛋白G（IgG）和免疫球蛋白E（IgE）免疫复合物刺激循环嗜酸性粒细胞和中性粒细胞释放过氧化氢（H2O2）的能力。PMA能够刺激这两种细胞类型产生显著的呼吸爆发，PMA诱导的嗜酸性粒细胞中H2O2的产生高于中性粒细胞（p小于0.05）。当细胞受到IgG刺激时也获得了类似的结果（p小于0.05）。相比之下，聚集的人骨髓瘤IgE仅能诱导外周血嗜酸性粒细胞释放可检测到的H2O2。我们得出结论，嗜酸性粒细胞释放H2O2可能在与嗜酸性粒细胞增多症相关的实质损伤以及疾病活动部位免疫复合物的存在中起作用。
